从一名貌似健康的男性供体的皮肤成纤维细胞中生成人诱导多能干细胞系 UNIGEi003-A。
Generation of human induced pluripotent stem cell line UNIGEi003-A from skin fibroblasts of an apparently healthy male donor.
机构信息
Laboratory of Tumor Immunology, Translational Research Center in Onco-Hematology, Department of Internal Medicine Specialties, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Vanarix SA, Lausanne, Switzerland.
出版信息
Stem Cell Res. 2020 Oct;48:101928. doi: 10.1016/j.scr.2020.101928. Epub 2020 Aug 3.
Dermal fibroblasts isolated from an apparently healthy 50-year-old man were successfully transformed into induced pluripotent stem cells (iPSCs) by using the integration-free CytoTune-iPS Sendai Reprogramming method. The generated iPSC line has been expanded under feeder-free conditions and displayed all hallmarks of a standard pluripotent stem cell line such as a normal karyotype, expression of pluripotent factors and differentiation capacity into the three germ layers.
从一位看似健康的 50 岁男性中分离出来的真皮成纤维细胞，通过使用无整合的 CytoTune-iPS Sendai 重编程方法，成功地被转化为诱导多能干细胞（iPSC）。生成的 iPSC 系在无饲养层条件下进行了扩增，并表现出标准多能干细胞系的所有特征，例如正常核型、多能因子的表达和向三个胚层的分化能力。
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