Serwin Karol, Kozak Małgorzata, Mazurek-Mochol Małgorzata, Piotrowska Katarzyna, Czerewaty Michał, Pawlik Andrzej
Department of Infectious, Tropical Diseases and Immune Deficiency, Pomeranian Medical University in Szczecin, Arkońska 4, 71-455 Szczecin, Poland.
Chair and Department of Dental Prosthetics, Pomeranian Medical University, Powstańców Wlkp 72, 70-111 Szczecin, Poland.
Immunobiology. 2023 Nov;228(6):152760. doi: 10.1016/j.imbio.2023.152760. Epub 2023 Nov 7.
Periodontitis is a local inflammatory reaction caused by bacterial infection in which immune cells, including macrophages, are involved. Recent studies have shown that an important regulator of macrophage function is the human macrophage immunometabolism regulator (MACIR). This gene has been shown to play a key role in modulating the immune response by affecting the activity of fibroblasts and macrophages. In this study, we investigated the expression of MACIR in the gingival tissues of patients with periodontal disease, as well as the effect of IL-1β and TNF-α on the expression of MACIR gene and protein in human gingival fibroblasts.
MACIR mRNA expression in gingival tissue samples was determined using Real-time PCR. Expression of MACIR protein was determined using immunofluorescent staining and western blotting.
The MACIR mRNA expression in gingival tissue samples in patients with periodontitis was statistically significantly lower than in gingival tissue samples from healthy controls (p = 0.009). The stimulation of human gingival fibroblasts with IL-1β and TNF-α resulted in a statistically significant decrease of MACIR gene mRNA expression. In western blotting and immunofluorescent analysis, we confirmed that the stimulation of the primary culture of human gingival fibroblasts by both IL-1β and TNF-α decreases the expression of MACIR protein.
The results of the study suggest that MACIR is an important regulator of the inflammatory process in patients with periodontitis. Decreased expression of the MACIR gene may activate macrophages to secrete mediators that increase inflammation and cause periodontal tissue destruction.
牙周炎是一种由细菌感染引起的局部炎症反应，其中包括巨噬细胞在内的免疫细胞参与其中。最近的研究表明，人类巨噬细胞免疫代谢调节因子（MACIR）是巨噬细胞功能的重要调节因子。该基因已被证明通过影响成纤维细胞和巨噬细胞的活性在调节免疫反应中起关键作用。在本研究中，我们调查了MACIR在牙周病患者牙龈组织中的表达，以及白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）对人牙龈成纤维细胞中MACIR基因和蛋白表达的影响。
使用实时聚合酶链反应（Real-time PCR）测定牙龈组织样本中MACIR mRNA的表达。使用免疫荧光染色和蛋白质印迹法测定MACIR蛋白的表达。
牙周炎患者牙龈组织样本中MACIR mRNA的表达在统计学上显著低于健康对照者的牙龈组织样本（p = 0.009）。用IL-1β和TNF-α刺激人牙龈成纤维细胞导致MACIR基因mRNA表达在统计学上显著降低。在蛋白质印迹和免疫荧光分析中，我们证实IL-1β和TNF-α对人牙龈成纤维细胞原代培养物的刺激均降低了MACIR蛋白的表达。
研究结果表明，MACIR是牙周炎患者炎症过程的重要调节因子。MACIR基因表达的降低可能会激活巨噬细胞分泌增加炎症并导致牙周组织破坏的介质。
