Department of Dental Medicine, Division of Oral Diseases, Karolinska Institutet, Huddinge, Sweden.
J Periodontol. 2020 Jun;91(6):828-835. doi: 10.1002/JPER.19-0296. Epub 2020 Jan 20.
Colony-stimulating factor 1 (CSF-1) and interleukin (IL)-34 are important for the functions of myeloid lineage cells and are involved in several chronic inflammatory conditions associated with tissue degeneration. The aim of this study is to evaluate the expression of CSF-1 and IL-34 in gingival tissue and gingival fibroblasts (GF) from patients with periodontitis and controls.
Gingival biopsies were obtained from 19 periodontitis patients and 15 controls. Expression of CSF-1 and IL-34 in gingival tissue was assessed by western blot and localization by immunohistochemistry. Expression of CSF1 and IL34 mRNA in GF was analyzed by real-time polymerase chain reaction and protein expression visualized by immunofluorescence stainings. CSF-1 and IL-34 secretion from GF was evaluated in response to tumor necrosis factor-alpha (TNF-α), IL-1β, Escherichia coli lipopolysaccharide (Ec-LPS) and Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) stimulation, using enzyme-linked immunosorbent assays.
CSF-1 was increased in gingival tissue from periodontitis patients compared with controls (P < 0.05) whereas IL-34 expression was similar. In GF from a non-periodontitis donor, stimulation with either TNF-α, IL-1β, Ec-LPS, or Pg-LPS, increased the secretion of CSF-1 (P < 0.05) and Ec-LPS stimulation increased IL-34 (P < 0.05). CSF-1 and IL-34 were expressed and secreted constitutively from GF, with comparable levels in GF from periodontitis patients and controls. Inflammatory stimuli increased the secretion of CSF-1 and IL-34 with comparable levels measured from GF from periodontitis patients and controls (P < 0.05).
The expression of CSF-1 and IL-34 in gingival tissue and fibroblasts suggests involvement in myeloid cell functions during periodontal inflammation.
集落刺激因子 1（CSF-1）和白细胞介素（IL）-34 对髓系细胞的功能很重要，并且与几种与组织退化相关的慢性炎症状态有关。本研究旨在评估牙周炎患者和对照者牙龈组织和牙龈成纤维细胞（GF）中 CSF-1 和 IL-34 的表达。
从 19 名牙周炎患者和 15 名对照者中获取牙龈活检。通过 Western blot 评估牙龈组织中 CSF-1 和 IL-34 的表达，并通过免疫组织化学进行定位。通过实时聚合酶链反应分析 GF 中 CSF1 和 IL34 mRNA 的表达，并通过免疫荧光染色可视化蛋白表达。通过酶联免疫吸附试验评估 GF 对肿瘤坏死因子-α（TNF-α）、IL-1β、大肠杆菌脂多糖（Ec-LPS）和牙龈卟啉单胞菌脂多糖（Pg-LPS）刺激的 CSF-1 和 IL-34 的分泌。
与对照组相比，牙周炎患者的牙龈组织中 CSF-1 增加（P<0.05），而 IL-34 表达相似。在非牙周炎供体的 GF 中，TNF-α、IL-1β、Ec-LPS 或 Pg-LPS 的刺激均增加 CSF-1 的分泌（P<0.05），并且 Ec-LPS 刺激增加 IL-34（P<0.05）。CSF-1 和 IL-34 从 GF 中持续表达和分泌，在牙周炎患者和对照组的 GF 中具有相似水平。炎症刺激物增加 CSF-1 和 IL-34 的分泌，从牙周炎患者和对照组的 GF 中测量到的水平相似（P<0.05）。
牙龈组织和成纤维细胞中 CSF-1 和 IL-34 的表达表明它们参与了牙周炎炎症期间髓系细胞的功能。
