Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161 Rome, Italy.
Faculty of Chemistry and Biology, Department of Materials Chemistry, University of Santiago of Chile, Av. Libertador Bernardo ÓHiggins 3363, 8320000 Estacion Central, Santiago, Chile.
Bioelectrochemistry. 2024 Apr;156:108590. doi: 10.1016/j.bioelechem.2023.108590. Epub 2023 Oct 30.
This work describes the development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care. The electrochemical platform was based on a graphene (GPH) screen-printed electrode (SPE) functionalized by gold-nanospheres (AuNSs) and antibodies against MMP-8 protein (anti-MMP-8). The functionalization with anti-MMP-8 was realized by using 11-mercaptoundecanoic acid (11-MUA), thanks to its ability to give strong sulfur bonds with its -SH end, and to cross-link the -NH groups of the antibody molecule with the other -COOH end, using the traditional EDC-NHS method. The voltammetric sensor showed good performances with a linear range of 2.5-300 ng mL, a LOD value of 1.0 ± 0.1 ng mL and a sensitivity of 0.05 µA mL cm ng. Moreover, the proposed immunosensor was tested in real saliva samples, showing comparable results to those obtained with the conventional ELISA method. The biosensor was single-use and cost-effective and required a small quantity of test medium and a short preparation time, representing a very attractive biosensor for MMP-8 detection in human saliva.
本工作开发了一种新型的伏安免疫传感器，用于在即时检测中检测唾液中的 MMP-8。电化学平台基于金纳米球（AuNSs）和针对 MMP-8 蛋白的抗体修饰的石墨烯（GPH）丝网印刷电极（SPE）。通过使用 11-巯基十一酸（11-MUA）实现了与抗 MMP-8 的功能化，这得益于其 -SH 端与强硫键结合的能力，并通过传统的 EDC-NHS 方法使抗体分子的 -NH 基团与另一个 -COOH 端交联。伏安传感器表现出良好的性能，线性范围为 2.5-300ngmL，LOD 值为 1.0±0.1ngmL，灵敏度为 0.05µA mL cm ng。此外，该免疫传感器在实际唾液样本中进行了测试，与传统的 ELISA 方法获得的结果相当。该生物传感器为一次性使用且具有成本效益，需要少量的测试介质和较短的制备时间，代表了一种非常有吸引力的用于检测人唾液中 MMP-8 的生物传感器。
