基于柠檬酸衍生碳点的抗坏血酸氧化酶样活性的果汁和饮料中抗坏血酸的荧光“开启”传感器。
A fluorescence "turn-on" sensor for ascorbic acid in fruit juice and beverage based on ascorbate oxidase-like activity of citric acid-derived carbon dots.
机构信息
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350122, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350122, China.
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350122, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350122, China; Department of Pharmaceutical Analysis, Faculty of Pharmacy, Quanzhou Medical College, Quanzhou 362011, China.
出版信息
Food Chem. 2024 Mar 30;437(Pt 2):137928. doi: 10.1016/j.foodchem.2023.137928. Epub 2023 Nov 7.
Citric acid-derived carbon dots (CA-CDs) without any modifications were found to have the ascorbate oxidase (AAO)-like activity. The CA-CDs have high affinity for ascorbic acid (AA), which is similar to natural AAO. The robustness of CA-CDs is greater than that of AAO. Based on the AAO mimetic activity of CA-CDs, a sensitive turn-on mode and natural enzyme-free fluorescence detection method has been developed for AA in some fruit juice and beverage samples with satisfied recoveries. This study provides CDs-based AAO mimetic nanozymes to replace the expensive natural enzymes or heavy metal-based nanozymes, which will show great potential in biological and food assays.
柠檬酸衍生的碳点（CA-CDs）无需任何修饰即可表现出抗坏血酸氧化酶（AAO）样活性。CA-CDs 对抗坏血酸（AA）具有高亲和力，这与天然 AAO 相似。CA-CDs 的稳健性强于 AAO。基于 CA-CDs 的 AAO 模拟活性，建立了一种灵敏的开环模式和无天然酶的荧光检测方法，用于检测一些果汁和饮料样品中的 AA，回收率令人满意。本研究为基于 CDs 的 AAO 模拟纳米酶替代昂贵的天然酶或基于重金属的纳米酶提供了可能，这将在生物和食品分析中显示出巨大的潜力。
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