U.S. Geological Survey Alaska Science Center, Anchorage, AK, USA.
U.S. Geological Survey Alaska Science Center, Anchorage, AK, USA.
Virology. 2024 Jan;589:109938. doi: 10.1016/j.virol.2023.109938. Epub 2023 Nov 10.
We detected and characterized highly pathogenic avian influenza viruses among hunter-harvested wild waterfowl inhabiting western Alaska during September-October 2022 using a molecular sequencing pipeline applied to RNA extracts derived directly from original swab samples. Genomic characterization of 10 H5 clade 2.3.4.4b avian influenza viruses detected with high confidence provided evidence for three independent viral introductions into Alaska. Our results highlight the utility and some potential limits of applying molecular processing approaches directly to RNA extracts from original swab samples for viral research and monitoring.
我们使用分子测序技术，对 2022 年 9 月至 10 月在阿拉斯加西部栖息的猎捕野生水鸟的原始拭子样本直接提取的 RNA 进行检测和分析，从中发现并鉴定了高致病性禽流感病毒。对 10 株 H5 分支 2.3.4.4b 禽流感病毒的高可信度基因组特征分析，为 3 种独立的病毒传入阿拉斯加提供了证据。我们的研究结果突出了直接对原始拭子样本 RNA 提取物应用分子处理方法进行病毒研究和监测的实用性及其潜在局限性。
