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[用同源放射免疫分析法测定大鼠血清中总三碘-L-甲状腺原氨酸]

[Measurement of total triiodo-L-thyronine in rat serum by homologous radioimmunoanalysis].

作者信息

Morell M, Fernández-Pastor V J

出版信息

Rev Esp Fisiol. 1986 Sep;42(3):383-7.

PMID:3797786
Abstract

A radioimmunoanalysis comparative study of total tri-iodo-L-thyronine patterns in human and rat sera revealed a quantitative difference between the amounts of labelled antigen which associated with the specific antibody in each case. At the same time, in another experiment, three different protein concentrations were dissolved in rat serum standards producing the results that, at lower protein concentrations a larger quantity of antigen/antibody complex was formed. These changes must be kept in mind for the L-T3 measurement in rat serum.

摘要

一项关于人和大鼠血清中总三碘-L-甲状腺原氨酸模式的放射免疫分析比较研究显示,在每种情况下,与特异性抗体结合的标记抗原量存在定量差异。同时,在另一项实验中,将三种不同的蛋白质浓度溶解在大鼠血清标准品中,结果表明,在较低蛋白质浓度下会形成大量的抗原/抗体复合物。在测定大鼠血清中的L-T3时,必须牢记这些变化。

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