Obregon M J, Morreale de Escobar G, Escobar del Rey F
Endocrinology. 1978 Dec;103(6):2145-53. doi: 10.1210/endo-103-6-2145.
The concentrations of T3 (3',3,5-triiodo-L-thyronine) have been determined by RIA in plasma, liver, kidney, heart, muscle, brain, and lungs of normal male rats. The method involves homogenization, addition of a high specific activity labeled T3 tracer, extraction with ethanol, separation by paper chromatography, elution of the labeled T3 spot, evaporation of an aliquot, and assay of its T3 content by a highly sensitive RIA. In order to validate the results thus obtained, they were compared with the T3 concentrations determined for the same samples using an isotopic equilibrium technique. The data obtained by RIA were in close agreement with those derived from the isotopic equilibrium technique, whenever the latter could be applied accurately. The present RIA method permits the determination of T3 in tissues without imposing the well known limitations of the isotopic equilibrium technique. The concentration of T3 was higher in the kidney than in the liver. In kidney, liver, brain, and lung, it was higher than the concentration in plasma. The concentration of T3 in heart was the same as that in plasma. Similarities and differences with respect to data reported by others are discussed.
采用放射免疫分析法（RIA）测定了正常雄性大鼠血浆、肝脏、肾脏、心脏、肌肉、大脑和肺组织中三碘甲状腺原氨酸（T3）的浓度。该方法包括匀浆、加入高比活度标记的T3示踪剂、用乙醇提取、纸层析分离、洗脱标记的T3斑点、取一份样品蒸发以及用高灵敏度放射免疫分析法测定其T3含量。为了验证所得结果，将其与使用同位素平衡技术测定的相同样品的T3浓度进行了比较。只要同位素平衡技术能够准确应用，放射免疫分析法获得的数据就与该技术得出的数据高度一致。目前的放射免疫分析法能够测定组织中的T3，而不存在同位素平衡技术众所周知的局限性。肾脏中T3的浓度高于肝脏。在肾脏、肝脏、大脑和肺中，T3的浓度高于血浆中的浓度。心脏中T3的浓度与血浆中的浓度相同。文中还讨论了与其他研究报告数据的异同。
