The University of Houston College of Optometry, Houston, TX, USA.
The University of Houston College of Optometry, Houston, TX, USA.
Cont Lens Anterior Eye. 2024 Feb;47(1):102090. doi: 10.1016/j.clae.2023.102090. Epub 2023 Nov 17.
Lid wiper epitheliopathy (LWE) is a marker of an abnormal lid/cornea interaction. This study proposes an automated Hue-Value grading algorithm of LWE staining following manual selection of the region of interest.
Images of LWE staining were processed using Hue and Value from HSV (Hue-Saturation-Value) color space with a custom MATLAB program. Thirty-one images were successfully analyzed. Examiners analyzed images in random order twice, separated by more than a week. Bland Altman and Intraclass Correlation Coefficients (ICC) were performed.
There was no difference (p > 0.05) between upper (UL) and lower (LL) eyelids for LWE height (UL: 0.12 ± 0.12 mm, LL: 0.12 ± 0.07 mm), width (UL: 10.70 ± 3.84 mm, LL: 10.26 ± 3.49 mm), or area (UL: 2.85 ± 2.67 mm, LL: 2.63 ± 1.71 mm). There was no between examiner difference for all eyelid LWE height or area (p > 0.05), but a difference in LWE width (0.16 mm; p = 0.031). ICC for LWE height, width and area were 0.996 (95% CI: 0.993 to 0.998), 0.997 (95% CI: 0.992 to 0.998) and 0.999 (95% CI: 0.998 to 0.999). There was no between examiner difference for height or area (p > 0.05) for UL, but a difference in LWE width (0.28 mm; p = 0.026). ICC for height, width and area were 0.999 (95% CI: 0.996 to 1.00), 0.995 (95% CI: 0.982 to 0.999) and 1.00 (95% CI: 0.999 to 1.00). There was no difference in LWE height, width or area for LL (all p > 0.05). ICC were 0.991 (95% CI: 0.973 to 0.997) for height, 0.998 (95% CI: 0.995 to 0.999) for width and 0.997 (95% CI: 0.990 to 0.999) for area.
This novel method results in highly repeatable interexaminer measures of LWE staining after general lid region delineation. Small differences in LWE width were observed between examiners.
睑缘刷状上皮病（Lid Wiper Epitheliopathy，LWE）是一种异常睑缘/角膜相互作用的标志物。本研究提出了一种自动的 HSV（Hue-Saturation-Value）颜色空间的 Hue-Value 分级算法，用于手动选择感兴趣区域。
使用 Hue 和 Value 对 LWE 染色图像进行处理，使用自定义的 MATLAB 程序进行 HSV 颜色空间处理。成功分析了 31 张图像。检查者在间隔超过一周的时间内两次随机分析图像。进行了 Bland Altman 和组内相关系数（Intraclass Correlation Coefficient，ICC）分析。
上睑（UL）和下睑（LL）的 LWE 高度（UL：0.12±0.12mm，LL：0.12±0.07mm）、宽度（UL：10.70±3.84mm，LL：10.26±3.49mm）和面积（UL：2.85±2.67mm，LL：2.63±1.71mm）之间没有差异（p>0.05）。对于所有眼睑的 LWE 高度或面积，检查者之间没有差异（p>0.05），但 LWE 宽度有差异（0.16mm；p=0.031）。LWE 高度、宽度和面积的 ICC 分别为 0.996（95%CI：0.993 至 0.998）、0.997（95%CI：0.992 至 0.998）和 0.999（95%CI：0.998 至 0.999）。对于 UL，高度和面积的检查者之间没有差异（p>0.05），但 LWE 宽度有差异（0.28mm；p=0.026）。高度、宽度和面积的 ICC 分别为 0.999（95%CI：0.996 至 1.00）、0.995（95%CI：0.982 至 0.999）和 1.00（95%CI：0.999 至 1.00）。对于 LL，LWE 高度、宽度或面积没有差异（均 p>0.05）。高度的 ICC 为 0.991（95%CI：0.973 至 0.997），宽度的 ICC 为 0.998（95%CI：0.995 至 0.999），面积的 ICC 为 0.997（95%CI：0.990 至 0.999）。
本研究提出的一种新方法可在眼睑一般区域划定后，对 LWE 染色进行高度重复的检查者间测量。检查者之间观察到 LWE 宽度的微小差异。
