The Eye Center, Southern College of Optometry Memphis, 1245 Madison Avenue, Memphis, TN 38104, USA.
Department of Vision & Hearing Sciences, Anglia Ruskin University, Cambridge, UK.
Cont Lens Anterior Eye. 2021 Jun;44(3):101332. doi: 10.1016/j.clae.2020.04.016. Epub 2020 May 14.
Lid wiper epitheliopathy (LWE) is a clinical sign that has been associated with dry eye disease. This study used a semi-automated method to identify the effect of drop instillation and post-dye viewing time on the absorption of lissamine green (LG) and sodium fluorescein (NaFl) on the upper eyelid in order to ascertain the optimal identification for LWE assessment.
In 37 participants with LWE, 1-drop of 1% LG (10 μL) was applied to the superior bulbar conjunctiva in the right eye, and photographs of the lid margin were taken 1, 3, and 5 min after instillation. Measurements were repeated in the same eye following instillations of 2-drops of 1% LG. The same procedures were followed for application of 2% NaFl (2 μL) to the left eye. Staining area was determined using software to detect and measure dye-stained images. Analysis used a linear mixed model with fixed effects of time, number of drops and their interaction.
For LG, multivariate analysis showed that time of drop instillation was significant (p = 0.0091) as was the area of staining in the 2-drop versus 1-drop condition (p < 0.0001). For NaFl, there was a significant effect of time (p < 0.0001), drops (p < 0.0001), and a time/drops interaction (p < 0.0134), suggesting that both time and number of drops are important.
A single drop of dye is insufficient to reveal the full extent of LWE staining. A 2-drop instillation is recommended and observation is recommended between 1 and 5 min (LG) and between 3 and 5 min (NaFl).
眼睑擦拭上皮病（LWE）是与干眼症相关的临床征象。本研究使用半自动方法来确定滴剂滴注和染色后观察时间对在上眼睑吸收丽丝胺绿（LG）和荧光素钠（NaFl）的影响，以确定用于 LWE 评估的最佳识别方法。
在 37 名患有 LWE 的参与者中，右眼的上球结膜滴加 1%LG（10 μL）1 滴，滴注后 1、3 和 5 分钟拍摄眼睑缘照片。在同一只眼中重复滴加 2 滴 1%LG 的相同程序。在左眼应用 2%NaFl（2 μL）时，遵循相同的程序。使用软件检测和测量染色图像来确定染色区域。分析使用具有时间、滴数及其相互作用的固定效果的线性混合模型。
对于 LG，多变量分析显示滴注时间具有显著影响（p = 0.0091），2 滴与 1 滴条件下的染色面积也具有显著影响（p < 0.0001）。对于 NaFl，时间（p < 0.0001）、滴数（p < 0.0001）和时间/滴数相互作用（p < 0.0134）均有显著影响，这表明时间和滴数都很重要。
一滴染料不足以揭示 LWE 染色的全部范围。建议滴加 2 滴，并建议在 1 至 5 分钟（LG）和 3 至 5 分钟（NaFl）之间观察。
