State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Department of Orthopedics, The First Hospital of Shanxi Medical University, Taiyuan 030000, China.
College of Animal Science, Shanxi Agricultural University, Taigu 030801, China.
Food Chem. 2024 Apr 16;438:137982. doi: 10.1016/j.foodchem.2023.137982. Epub 2023 Nov 13.
Fecal samples from 20 healthy adults were collected for in vitro fermentation experiments to investigate the effects of combined probiotics on the utilization of grape seed extract in humans. After fermenting for 24 h, short-chain fatty acids, metabolites, and gut microbiota composition were analyzed. Short-chain fatty acids in the grape seed extract probiotics group were significantly higher than those in the grape seed extract group. Probiotics significantly enhanced the conversion and utilization of catechins and epicatechins in grape seed extract group and increased the production of 3-hydroxyphenylacetic acid. The 16S rRNA sequencing results revealed that compound probiotics significantly increased the relative abundance of Lacticaseibacillus, HT002, Bifidobacterium, and Lactobacillus and reduced that of Escherichia-Shigella. Our findings showed considerable individual variability in the metabolic utilization of grape seed extract in humans. The consumption of probiotics appears to significantly enhance the utilization.
收集 20 名健康成年人的粪便样本进行体外发酵实验，以研究复合益生菌对人体葡萄籽提取物利用的影响。发酵 24 小时后，分析短链脂肪酸、代谢物和肠道微生物组成。葡萄籽提取物益生菌组的短链脂肪酸明显高于葡萄籽提取物组。益生菌显著提高了葡萄籽提取物组儿茶素和表儿茶素的转化率和利用率，并增加了 3-羟基苯乙酸的生成。16S rRNA 测序结果表明，复合益生菌显著增加了乳酸乳球菌、HT002、双歧杆菌和乳酸菌的相对丰度，降低了大肠埃希菌-志贺菌的相对丰度。我们的研究结果表明，人体对葡萄籽提取物的代谢利用存在相当大的个体差异。益生菌的摄入似乎显著提高了利用率。
