Mi Yuwei, Lin Chen, Zheng Huowang, Wu Ying, Hou Yanbin, Hu Jieqiong, Mao Jiaxin, Dai Ni, Li Xiaoqiong, Lou Zhongze, Ji Yunxin
Department of Psychosomatic Medicine, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Front Nutr. 2025 Apr 28;12:1587742. doi: 10.3389/fnut.2025.1587742. eCollection 2025.
Previous studies have found that Polysaccharide (APS) and PS128 (PS128) have potential antidepressant effects, but their effects on the gut microbiota and metabolites of major depressive disorder (MDD) are still unclear. We examined the effect of APS on gut microbiota and metabolites of first-episode and drug naïve MDD patients using fermentation, and further explored whether PS128 could enhance the utilization ability of APS. Fresh fecal samples from 15 MDD patients were collected, and analyzed for differences in gas production, gut microbiota, and tryptophan (Trp) related metabolites after 48 h of fermentation. APS fermentation increased the abundance of and decreased the abundance of ( < 0.05). APS also increased total gas production and levels of indole lactic acid (ILA), Trp, and 5-hydroxytryptophan (5-HTP) ( < 0.05). Compared with APS, APS with PS128 synbiotics fermentation increased the abundance of ( < 0.05), reduced total gas production and percentages of CO, H, and HS ( < 0.05), and to some extent increased the levels of ILA, Trp, and 5-HTP, although not statistically significant ( > 0.05). Correlation analysis showed was positively correlated with ILA, Trp and 5-HTP; On the contrary, was negatively correlated with ILA, Trp, and 5-HTP. All these results suggest that APS could regulate gut microbiota structure and Trp related metabolites in MDD patients; Compared to APS, APS and PS128 synbiotic fermentation could reduce gas production but shows limited ability to modulate gut microbiota structure or Trp related metabolites in MDD patients.
先前的研究发现，多糖（APS）和PS128具有潜在的抗抑郁作用，但其对重度抑郁症（MDD）患者肠道微生物群和代谢产物的影响仍不清楚。我们通过发酵研究了APS对首发未用药MDD患者肠道微生物群和代谢产物的影响，并进一步探讨了PS128是否能增强APS的利用能力。收集了15例MDD患者的新鲜粪便样本，分析发酵48小时后产气、肠道微生物群和色氨酸（Trp）相关代谢产物的差异。APS发酵增加了[具体菌种1]的丰度，降低了[具体菌种2]的丰度（P<0.05）。APS还增加了总产气量以及吲哚乳酸（ILA）、Trp和5-羟色氨酸（5-HTP）的水平（P<0.05）。与APS相比，APS与PS128合生元发酵增加了[具体菌种3]的丰度（P<0.05），降低了总产气量以及CO、H和HS的百分比（P<0.05），并在一定程度上增加了ILA、Trp和5-HTP的水平，尽管无统计学意义（P>0.05）。相关性分析表明，[具体菌种1]与ILA、Trp和5-HTP呈正相关；相反，[具体菌种2]与ILA、Trp和5-HTP呈负相关。所有这些结果表明，APS可以调节MDD患者的肠道微生物群结构和Trp相关代谢产物；与APS相比，APS和PS128合生元发酵可以减少产气量，但调节MDD患者肠道微生物群结构或Trp相关代谢产物的能力有限。
