Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Int J Biol Macromol. 2024 Jan;255:128106. doi: 10.1016/j.ijbiomac.2023.128106. Epub 2023 Nov 17.
Long noncoding RNAs (lncRNAs) are transcribed in complex, overlapping, sense- and antisense orientations from intronic and intergenic regions of mammalian genomes. Transcription of genome in mammalian testis is more widespread compared to other organs. LncRNAs are involved in gene expression, chromatin regulation, mRNA stability and translation of proteins during diverse cellular functions. We report molecular cloning of two novel lncRNAs (mLINC-RBE and mLINC-RSAS) and their expression by RT-PCR as well as cellular localization by RNA in-situ hybridization in the mouse testes. mLINC-RBE is an intergenic lncRNA from chromosome 4, with 16.96 % repeat sequences, expressed as a sense transcript with piRNA sequences and its expression is localized into primary spermatocytes. mLINC-RSAS is an intergenic lncRNA from chromosome 2, with 49.7 % repeat sequences, expressed as both sense- and antisense transcripts with miRNA sequences and its expression is localized into different cell types, such as Sertoli cells, primary spermatocytes and round spermatids. The lncRNAs also contain sequences for some short peptides (micropeptides). This suggests that these two repeat sequence containing, intergenic genomic sense- and antisense transcripts expressed as lncRNAs with piRNAs, miRNAs, and showing cell-type specific, differential expression may regulate important functions in mammalian testes. Such functions may be regulated by RNA structures, RNA processing and RNA-protein complexes.
长链非编码 RNA（lncRNA）是从哺乳动物基因组的内含子和基因间区以复杂、重叠、有义及反义的方向转录而来的。与其他器官相比，哺乳动物睾丸中的基因组转录更为广泛。lncRNA 参与了不同细胞功能中基因表达、染色质调控、mRNA 稳定性和蛋白质翻译等过程。我们报告了两个新的 lncRNA（mLINC-RBE 和 mLINC-RSAS）的分子克隆及其通过 RT-PCR 的表达，以及通过 RNA 原位杂交在小鼠睾丸中的细胞定位。mLINC-RBE 是来自 4 号染色体的基因间 lncRNA，具有 16.96%的重复序列，作为具有 piRNA 序列的有义转录本表达，其表达定位于初级精母细胞中。mLINC-RSAS 是来自 2 号染色体的基因间 lncRNA，具有 49.7%的重复序列，作为具有 miRNA 序列的有义和反义转录本表达，其表达定位于不同的细胞类型，如支持细胞、初级精母细胞和圆形精子细胞。这些 lncRNA 还包含一些短肽（微肽）的序列。这表明这两个含有重复序列、基因间的有义及反义转录本作为具有 piRNA、miRNA 的 lncRNA 表达，并显示出细胞类型特异性、差异表达，可能在哺乳动物睾丸中调节重要功能。这些功能可能受到 RNA 结构、RNA 加工和 RNA-蛋白质复合物的调节。
