Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, PA, 15224, USA.
Biogerontology. 2024 Nov;25(6):1053-1067. doi: 10.1007/s10522-024-10119-5. Epub 2024 Jul 17.
Long noncoding RNAs (lncRNAs) are important regulatory biomolecules responsible for many cellular processes. The aging of mammals is manifested by a slow and gradual decline of physiological functions after adulthood, progressively resulting in age-related diseases. Testis comprises different cell-types with defined functions for producing haploid gametes and androgens in males, contributing gene-pool to the next generation with genetic variations to species for evolutionary advantage. The LINC-RBE (long intergenic noncoding-rat brain expressed) RNA showed highest expression in the Leydig cells, responsible for steroidogenesis and production of testosterone; higher expression in primary spermatocytes (pachytene cells), responsible for generation of haploid gametes and high expression in Sertoli cells, the nursing cells of the testes. Testes of immature (4-weeks), adult (16- and 44-weeks), and nearly-old (70-weeks) rats showed low, high, and again low levels of expression, respectively. This along with the nuclear-cytoplasmic localization of LINC-RBE RNA showed age-related expression and function. Thus, expression of LINC-RBE is involved in the molecular physiology of testes, especially Leydig cells, primary spermatocytes, and Sertoli cells. The decline in its expression correlates with diminishing reproductive function of the testes during aging of the rat.
长链非编码 RNA（lncRNAs）是重要的调节生物分子，负责许多细胞过程。哺乳动物的衰老表现为成年后生理功能的缓慢而逐渐下降，逐渐导致与年龄相关的疾病。睾丸包含不同的细胞类型，具有产生雄性单倍体配子和雄激素的特定功能，为下一代提供基因库，通过遗传变异为物种带来进化优势。LINC-RBE（长基因间非编码-大鼠脑表达）RNA 在睾丸间质细胞中的表达最高，间质细胞负责类固醇生成和睾酮的产生；在初级精母细胞（粗线期细胞）中的表达较高，负责产生单倍体配子，在支持细胞中的表达较高，支持细胞是睾丸的滋养细胞。未成熟（4 周）、成年（16 周和 44 周）和近老年（70 周）大鼠的睾丸显示出低、高和再次低的表达水平。这与 LINC-RBE RNA 的核质定位一起显示出与年龄相关的表达和功能。因此，LINC-RBE 的表达参与睾丸的分子生理学，特别是睾丸间质细胞、初级精母细胞和支持细胞。其表达的下降与大鼠衰老过程中睾丸生殖功能的下降相关。
