Gupta S K, Talwar G P
Scand J Clin Lab Invest. 1986 Dec;46(8):751-9. doi: 10.3109/00365518609084047.
Two simple solid-phase sandwich immunoassays for human chorionic gonadotropin (hCG) employing monoclonal antibodies have been described. One is a sandwich erythro-immunoassay employing V-shaped well microtitration plates coated with monoclonal anti-beta-hCG antibody and monoclonal anti-alpha-hCG antibody labelled sheep erythrocytes. The second is a 'dot' enzyme immuno-assay employing dip-stick (plastic strips pasted with nitrocellulose pads) coated with monoclonal anti-beta-hCG antibody. Anti-alpha-hCG monoclonal-alkaline phosphatase conjugate was used to reveal hCG bound to solid surface. The assays can be performed by 'one-step' or 'two-step' procedures. Erythro-immunoassay as well as 'dot' enzyme immunoassay was able to detect in urine as low as 10 mIU hCG/ml. A good correlation was observed between the values obtained by these two methods as well as 'two-step' sandwich enzyme immunoassay on 47 urine samples.
已经描述了两种使用单克隆抗体的用于人绒毛膜促性腺激素（hCG）的简单固相夹心免疫测定法。一种是夹心红细胞免疫测定法，采用涂有抗β-hCG单克隆抗体和标记有羊红细胞的抗α-hCG单克隆抗体的V形孔微量滴定板。第二种是“斑点”酶免疫测定法，采用涂有抗β-hCG单克隆抗体的试纸条（粘贴有硝酸纤维素垫的塑料条）。抗α-hCG单克隆碱性磷酸酶结合物用于显示与固体表面结合的hCG。这些测定可以通过“一步”或“两步”程序进行。红细胞免疫测定法以及“斑点”酶免疫测定法能够检测到低至10 mIU hCG/ml的尿液。在这两种方法以及对47份尿液样本进行的“两步”夹心酶免疫测定法所获得的值之间观察到了良好的相关性。
