Harbin University, Harbin 150086, China.
College of Life Science, Northeast Agricultural University, Harbin 150030, China.
Bioresour Technol. 2024 Feb;393:130022. doi: 10.1016/j.biortech.2023.130022. Epub 2023 Nov 17.
The aim of this study was to compare the effect of functional inoculant and different nitrogen sources on the relationship among lignocellulose, precursors, and humus as well as their interactions with bacterial genera in straw composting. Results showed that inoculation improved the heating process and retained more nitrate compared to control. Inoculation increased the degradation of lignocellulosic components by 26.9%-81.6% and the formation of humus by 15.7%-23.0%. Bioinformatics analysis showed that inoculation enriched key genera Chryseolinea in complex nitrogen source (pig manure) compost and Pusillimas, Luteimonas, and Flavobacteria in single nitrogen source (urea) compost, which were related to humus formation. Network analysis found that inoculation and urea addition improved the microbial synergistic effect and inoculation combined with pig manure had more complex modularity and interactions. Combining the functional bacterial inoculant with urea helped to enhance the degradation of lignocellulose and humification process during straw composting especially with single nitrogen source.
本研究旨在比较功能接种剂和不同氮源对秸秆堆肥中木质纤维素、前体物和腐殖质之间关系及其与细菌属相互作用的影响。结果表明，接种剂改善了加热过程，并比对照保留了更多的硝酸盐。接种剂使木质纤维素成分的降解提高了 26.9%-81.6%，腐殖质的形成提高了 15.7%-23.0%。生物信息学分析表明，接种剂富集了复杂氮源（猪粪）堆肥中的关键属 Chryseolinea 和单一氮源（尿素）堆肥中的 Pusillimas、Luteimonas 和 Flavobacteria，这些与腐殖质的形成有关。网络分析发现，接种和尿素的添加改善了微生物协同作用，接种与猪粪的结合具有更复杂的模块性和相互作用。将功能细菌接种剂与尿素结合使用有助于提高秸秆堆肥过程中木质纤维素的降解和腐殖化过程，特别是在使用单一氮源时。
