Laboratory of Mechanistic Glycobiology, Department of Chemistry, Michigan Technological University, Houghton, MI, United States; Health Research Institute, Michigan Technological University, Houghton, MI, United States.
Laboratory of Mechanistic Glycobiology, Department of Chemistry, Michigan Technological University, Houghton, MI, United States.
Adv Carbohydr Chem Biochem. 2023;84:1-21. doi: 10.1016/bs.accb.2023.10.004. Epub 2023 Nov 10.
Lectins are predominantly oligomeric proteins with several binding sites per molecule. Glycoconjugates are their natural ligands, which often possess multiple binding epitopes. Thus, lectin-glycoconjugate interactions are mostly multivalent in nature. The mechanism of multivalent binding is fundamentally different from those described for monovalent interactions in textbooks and research papers. Over the years, binding studies that make use of different lectins and a variety of multivalent glycoconjugate ligands were conducted in order to understand the underlying principles of multivalency. Starting with seemingly simple synthetic multivalent analogs, systematic studies were carried out using natural glycoconjugate ligands with increasing valency and complexity. Those ligands included multivalent glycoproteins, polyvalent polysaccharides, including glycosaminoglycans, as well as supra-valent mucins and proteoglycans. Models and mechanisms of multivalent binding derived from quantitative data are summarized in the present updated review.
凝集素主要是具有多个结合位点的寡聚蛋白。糖缀合物是它们的天然配体，通常具有多个结合表位。因此，凝集素-糖缀合物相互作用在本质上大多是多价的。多价结合的机制与教科书中和研究论文中描述的单价相互作用的机制有根本的不同。多年来，利用不同的凝集素和各种多价糖缀合物配体进行了结合研究，以了解多价性的基本原理。从看似简单的合成多价类似物开始，使用具有增加的价数和复杂性的天然糖缀合物配体进行了系统的研究。这些配体包括多价糖蛋白、多价多糖，包括糖胺聚糖，以及超价粘蛋白和蛋白聚糖。从定量数据得出的多价结合模型和机制在本更新综述中进行了总结。
