Douthwaite S, Garrett R A, Wagner R, Feunteun J
Nucleic Acids Res. 1979 Jun 11;6(7):2453-70. doi: 10.1093/nar/6.7.2453.
An RNA fragment, constituting three subfragments of nucleotide sequences 1-11, 69-87 and 89-120, is the most ribonuclease-resistant part of the native 5S RNA of Escherichia coli, at 0 degrees C. A smaller fragment of nucleotide sequence 69-87 and 90-110 is ribonuclease-resistant at 25 degrees. Degradation of the L25-5S RNA complex with ribonuclease A or T2 yielded RNA fragments similar to those of the free 5S RNA at 0 degrees C and 25 degrees C; moreover L25 remained strongly bound to both RNA fragments and also produced some opening of the RNA structure in at least two positions. Protein L18 initially protected most of the 5S RNA against ribonuclease digestion, at 0 degrees C, but was then gradually released prior to the formation of the larger RNA fragment. It cannot be concluded, therefore, as it was earlier (Gray et al., 1973), that this RNA fragment contains the primary binding site of L18.
一个由核苷酸序列1 - 11、69 - 87和89 - 120的三个亚片段组成的RNA片段，在0℃时是大肠杆菌天然5S RNA中最抗核糖核酸酶的部分。核苷酸序列69 - 87和90 - 110的一个较小片段在25℃时抗核糖核酸酶。用核糖核酸酶A或T2降解L25 - 5S RNA复合物，在0℃和25℃时产生的RNA片段与游离5S RNA的片段相似；此外，L25仍与两个RNA片段紧密结合，并且在至少两个位置使RNA结构发生了一些开放。蛋白质L18最初在0℃时保护大部分5S RNA不被核糖核酸酶消化，但在形成较大RNA片段之前逐渐释放。因此，不能像早期（格雷等人，1973年）那样得出结论，即这个RNA片段包含L18的主要结合位点。
