对源自大肠杆菌5S RNA的一个片段进行的小角X射线散射研究。
A small angle x-ray scattering study of a fragment derived from E. coli 5S RNA.
作者信息
Leontis N B, Moore P B
出版信息
Nucleic Acids Res. 1984 Feb 24;12(4):2193-203. doi: 10.1093/nar/12.4.2193.
Abstract
The structure of a 62 base nuclease resistant fragment of E. coli 5S RNA (bases 1-11, 69-87, 89-120) has been examined by small angle x-ray scattering. The results obtained are indistinguishable from those expected if this oligonucleotide complex were a perfect RNA double helix of about 30 base pairs. These results indicate that this portion of 5S RNA is in a configuration which is approximately double helical, even though proper base pairing is possible over only half its length.
摘要
通过小角X射线散射研究了大肠杆菌5S RNA的一个62个碱基的核酸酶抗性片段（碱基1 - 11、69 - 87、89 - 120）的结构。如果该寡核苷酸复合物是一个约30个碱基对的完美RNA双螺旋，所获得的结果与预期结果没有区别。这些结果表明，5S RNA的这一部分处于近似双螺旋的构型，尽管仅在其长度的一半范围内可能存在正确的碱基配对。
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