Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, S7N 0X2, Canada.
Theor Appl Genet. 2023 Nov 20;136(12):249. doi: 10.1007/s00122-023-04483-y.
Two major quantitative trait loci (QTLs) and five minor QTLs for 10 pathotypes were identified on chromosomes C01, C03, C04 and C08 through genotyping-by-sequencing from Brassica oleracea. Clubroot caused by Plasmodiophora brassicae is an important disease in brassica crops. Managing clubroot disease of canola on the Canadian prairie is challenging due to the continuous emergence of new pathotypes. Brassica oleracea is considered a major source of quantitative resistance to clubroot. Genotyping-by-sequencing (GBS) was performed in the parental lines; T010000DH3 (susceptible), ECD11 (resistant) and 124 BC plants. A total of 4769 high-quality polymorphic SNP loci were obtained and distributed on 9 chromosomes of B. oleracea. Evaluation of 124 BCS lines for resistance to 10 pathotypes: 3A, 2B, 5C, 3D, 5G, 3H, 8J, 5K, 5L and 3O of P. brassicae, was carried out. Seven QTLs, 5 originating from ECD11 and 2 from T010000DH3, were detected. One major QTL designated as Rcr_C03-1 on C03 contributed 16.0-65.6% of phenotypic variation explained (PVE) for 8 pathotypes: 2B, 5C, 5G, 3H, 8J, 5K, 5L and 3O. Another major QTL designated as Rcr_C08-1 on C08 contributed 8.3 and 23.5% PVE for resistance to 8J and 5K, respectively. Five minor QTLs designated as Rcr_C01-1, Rcr_C03-2, Rcr_C03-3, Rcr_C04-1 and Rcr_C08-2 were detected on chromosomes C01, C03, C04 and C08 that contributed 8.3-23.5% PVE for 5 pathotypes each of 3A, 2B, 3D, 8J and 5K. There were 1, 10 and 4 genes encoding TIR-NBS-LRR/CC-NBS-LRR class disease resistance proteins in the Rcr_C01-1, Rcr_C03-1 and Rcr_C08-1 flanking regions. The syntenic regions of the two major QTLs Rcr_C03-1 and Rcr_C08-1 in the B. rapa genome 'Chiifu' were searched.
两个主要的数量性状位点（QTL）和五个次要的 QTL 被鉴定为甘蓝型油菜中的 10 种病原菌型的 C01、C03、C04 和 C08 染色体上的数量性状位点。由根肿菌引起的根肿病是十字花科作物的一种重要病害。由于新病原菌型的不断出现，管理加拿大草原油菜的根肿病具有挑战性。甘蓝型油菜被认为是根肿病的主要数量抗性来源。对亲本系 T010000DH3（敏感）、ECD11（抗性）和 124 BC 植物进行了测序分型（GBS）。共获得 4769 个高质量的多态性 SNP 位点，分布在 B.oleracea 的 9 条染色体上。对 124 个 BCS 系进行了对 10 种病原菌型（3A、2B、5C、3D、5G、3H、8J、5K、5L 和 3O）的抗性评估。检测到 7 个 QTL，其中 5 个来源于 ECD11，2 个来源于 T010000DH3。一个主要的 QTL 被命名为 C03 上的 Rcr_C03-1，它对 8 种病原菌型（2B、5C、5G、3H、8J、5K、5L 和 3O）的表型变异解释（PVE）贡献了 16.0-65.6%。另一个主要的 QTL 被命名为 C08 上的 Rcr_C08-1，它对 8J 和 5K 的抗性分别贡献了 8.3 和 23.5%的 PVE。在 C01、C03、C04 和 C08 染色体上检测到 5 个次要的 QTL，分别命名为 Rcr_C01-1、Rcr_C03-2、Rcr_C03-3、Rcr_C04-1 和 Rcr_C08-2，它们对 3A、2B、3D、8J 和 5K 的每种病原菌型的 PVE 贡献了 8.3-23.5%。在 Rcr_C01-1、Rcr_C03-1 和 Rcr_C08-1 的侧翼区域，有 1 个、10 个和 4 个编码 TIR-NBS-LRR/CC-NBS-LRR 类抗病蛋白的基因。在白菜基因组 'Chiifu' 中搜索了两个主要的 QTL Rcr_C03-1 和 Rcr_C08-1 的同源区域。
