Hosseinzadeh Shahram, Masoudi Ali Akbar
Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Genome. 2024 Mar 1;67(3):78-89. doi: 10.1139/gen-2023-0055. Epub 2023 Nov 20.
Sheep is the primary source of animal protein in Iran. Birth type is one of the significant features that determine total meat output. Little is known about how long non-coding RNAs (LncRNAs) affect litter size. The purpose of this research is to investigate the DE-LncRNAs in ovarian tissue between multiparous and uniparous Shal ewes. Through bioinformatics analyses, LncRNAs with variable expression levels between ewes were discovered. Target genes were annotated using the DAVID database, and STRING and Cytoscape software were used to evaluate their interactions. The expression levels of 148 LncRNAs were different in the multiparous and uniparous ewe groups (false discovery rate (FDR) < 0.05). Eight biological process terms, nine cellular component terms, 10 molecular function terms, and 38 KEGG pathways were significant (FDR < 0.05) in the GO analysis. One of the most significant processes impacting fertility is mitogen-activated protein kinase (MAPK) signaling pathway, followed by oocyte meiosis, gonadotropin-releasing hormone signaling pathway, progesterone-mediated oocyte maturation, oxytocin signaling pathway, and cAMP signaling pathway. ENSOARG00000025710, ENSOARG00000025667, ENSOARG00000026034, and ENSOARG00000026632 are LncRNAs that may affect litter size and fertility. The most crucial hub genes include , , , , , , and . We hope that this study will encourage researchers to further investigate the effect of LncRNAs on fertility.
绵羊是伊朗动物蛋白的主要来源。出生类型是决定总肉产量的重要特征之一。关于长链非编码RNA（LncRNAs）如何影响产仔数，人们知之甚少。本研究的目的是调查多胎和单胎沙勒母羊卵巢组织中的差异表达LncRNAs。通过生物信息学分析，发现了母羊之间表达水平不同的LncRNAs。使用DAVID数据库对靶基因进行注释，并使用STRING和Cytoscape软件评估它们的相互作用。148个LncRNAs的表达水平在多胎和单胎母羊组中存在差异（错误发现率（FDR）<0.05）。在GO分析中，8个生物学过程术语、9个细胞成分术语、10个分子功能术语和38个KEGG通路具有显著性（FDR<0.05）。影响生育能力最重要的过程之一是丝裂原活化蛋白激酶（MAPK）信号通路，其次是卵母细胞减数分裂、促性腺激素释放激素信号通路、孕酮介导的卵母细胞成熟、催产素信号通路和cAMP信号通路。ENSOARG00000025710、ENSOARG00000025667、ENSOARG00000026034和ENSOARG00000026632是可能影响产仔数和生育能力的LncRNAs。最关键的枢纽基因包括 、 、 、 、 、 和 。我们希望这项研究将鼓励研究人员进一步研究LncRNAs对生育能力的影响。
