Sorensen E M
Toxicol Lett. 1986 Dec;34(2-3):277-86. doi: 10.1016/0378-4274(86)90220-1.
This study was undertaken to quantitate morphological changes in primary cultures of liver cells exposed to benoxaprofen and to compare morphological assessments with standard assessments of functional integrity. Rat hepatocytes were exposed for 12 h to 0, 100, 500, or 1000 microM concentrations of benoxaprofen and subjected to double-blind morphometric analysis. As benoxaprofen concentration was increased from 0 to 1000 microM, the relative percentage of morphologically normal cells was reduced, whereas the percentages of damaged cell types increased. These results paralleled those obtained from functional assessments. Therefore, the use of morphometric techniques provided a good assessment of benoxaprofen-induced cytotoxicity and demonstrated the value of quantitative estimates in the evaluation of cellular injury.
本研究旨在定量分析暴露于贝诺洛芬的肝细胞原代培养物中的形态学变化，并将形态学评估与功能完整性的标准评估进行比较。将大鼠肝细胞暴露于0、100、500或1000微摩尔浓度的贝诺洛芬中12小时，然后进行双盲形态计量分析。随着贝诺洛芬浓度从0增加到1000微摩尔，形态正常细胞的相对百分比降低，而受损细胞类型的百分比增加。这些结果与功能评估的结果相似。因此，形态计量技术的应用为贝诺洛芬诱导的细胞毒性提供了良好的评估，并证明了定量估计在细胞损伤评估中的价值。
