Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin, 150030, P. R. China.
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, 08826, South Korea.
Adv Sci (Weinh). 2024 Jan;11(2):e2307505. doi: 10.1002/advs.202307505. Epub 2023 Nov 20.
In mice, retrotransposon-associated long noncoding RNAs (lncRNA) play important regulatory roles in pre-implantation development; however, it is largely unknown whether they function in the pre-implantation development in pigs. The current study aims to screen for retrotransposon-associated lncRNA in porcine early embryos and identifies a porcine 8-cell embryo-specific SINE-associated nuclear long noncoding RNA named SAWPA. SAWPA is essential for porcine embryonic development as depletion of SAWPA results in a developmental arrest at the 8-cell stage, accompanied by the inhibition of the JNK-MAPK signaling pathway. Mechanistically, SAWPA works in trans as a transcription factor for JNK through the formation of an RNA-protein complex with HNRNPA1 and MED8 binding the SINE elements upstream of JNK. Therefore, as the first functional SINE-associated long noncoding RNAs in pigs, SAWPA provides novel insights for the mechanism research on retrotransposons in mammalian pre-implantation development.
在小鼠中，逆转录转座子相关的长非编码 RNA（lncRNA）在着床前发育中发挥重要的调控作用；然而，它们在猪的着床前发育中是否发挥功能很大程度上是未知的。本研究旨在筛选猪早期胚胎中的逆转录转座子相关 lncRNA，并鉴定出一种猪 8 细胞胚胎特异性 SINE 相关核长非编码 RNA，命名为 SAWPA。SAWPA 对于猪胚胎发育是必需的，因为 SAWPA 的耗竭导致胚胎发育在 8 细胞阶段停滞，伴随着 JNK-MAPK 信号通路的抑制。从机制上讲，SAWPA 作为 JNK 的转录因子发挥作用，通过与 HNRNPA1 和 MED8 形成 RNA-蛋白质复合物，结合 JNK 上游的 SINE 元件。因此，作为猪中第一个功能性 SINE 相关长非编码 RNA，SAWPA 为哺乳动物着床前发育中逆转录转座子的机制研究提供了新的见解。
