Bharat Bansod Sneha, Deepak Thirumalai, Babu Anju R
Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, 769008, Odisha, India.
Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, 769008, Odisha, India.
Med Eng Phys. 2023 Nov;121:104061. doi: 10.1016/j.medengphy.2023.104061. Epub 2023 Oct 12.
Millions of people globally suffer from issues related to chronic wounds due to infection, burn, obesity, and diabetes. Nanocomposite with antibacterial and anti-inflammatory properties is a promising material to promote wound healing. This investigation primarily aims to synthesize reduced graphene oxide and titanium dioxide (rGO@TiO) nanocomposite for wound healing applications. The rGO@TiO nanocomposite was synthesized by the one-step hydrothermal technique, and the physicochemical characterization of synthesized nanocomposite was performed by X-ray diffraction, Fourier transforms infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy, and dynamic light scattering. Further, the nanocomposite antibacterial, cytotoxicity, and wound-healing properties were analyzed by disc diffusion method, MTT assay, and in vitro scratch assay, respectively. Based on the TEM images, the average particle size of TiO nanoparticles was around 9.26 ± 1.83 nm. The characteristics peak of Ti-O-Ti bonds was observed between 500 and 850 cm in the Fourier transforms infrared spectrum. The Raman spectrum of graphene oxide (GO) was obtained for bands D and G at 1354 cm and at 1593 cm, respectively. This GO peak intensity was reduced in rGO, revealing the oxygen functional group reduction. Moreover, the rGO@TiO nanocomposite exhibited dose-dependent antibacterial properties against the positive and negative bacterium. The cytotoxicity for 5-100 µg/mL of rGO@TiO nanocomposite was above the half-maximal inhibitory concentration value. The in vitro scratch assay for rGO@TiO indicates that the nanocomposite promotes cell proliferation and migration. The nanocomposite recovered the wound within 48 h. The rGO@TiO nanocomposite shows potential materials for wound healing applications.
全球数百万人因感染、烧伤、肥胖和糖尿病等问题患有与慢性伤口相关的疾病。具有抗菌和抗炎特性的纳米复合材料是促进伤口愈合的一种有前途的材料。本研究主要旨在合成用于伤口愈合应用的还原氧化石墨烯和二氧化钛（rGO@TiO）纳米复合材料。通过一步水热法合成了rGO@TiO纳米复合材料，并通过X射线衍射、傅里叶变换红外光谱、拉曼光谱、扫描电子显微镜、透射电子显微镜（TEM）、能量色散X射线光谱和动态光散射对合成的纳米复合材料进行了物理化学表征。此外，分别通过纸片扩散法、MTT法和体外划痕试验分析了纳米复合材料的抗菌、细胞毒性和伤口愈合特性。基于TEM图像，TiO纳米颗粒的平均粒径约为9.26±1.83nm。在傅里叶变换红外光谱中，在500至850cm之间观察到Ti-O-Ti键的特征峰。氧化石墨烯（GO）的拉曼光谱分别在1354cm和1593cm处获得了D带和G带。rGO中该GO峰强度降低，表明氧官能团减少。此外，rGO@TiO纳米复合材料对阳性和阴性细菌均表现出剂量依赖性抗菌特性。5-100μg/mL的rGO@TiO纳米复合材料的细胞毒性高于半数最大抑制浓度值。rGO@TiO的体外划痕试验表明，该纳米复合材料促进细胞增殖和迁移。该纳米复合材料在48小时内愈合了伤口。rGO@TiO纳米复合材料显示出用于伤口愈合应用的潜在材料。
