Zhou Jianle, Hollmann Frank, He Qi, Chen Wen, Ma Yunjian, Wang Yonghua
School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China.
Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ, Delft, The Netherlands.
ChemSusChem. 2024 Feb 8;17(3):e202301326. doi: 10.1002/cssc.202301326. Epub 2023 Nov 29.
The realm of photobiocatalytic alkane biofuel synthesis has burgeoned recently; however, the current dearth of well-established and scalable production methodologies in this domain remains conspicuous. In this investigation, we engineered a modified form of membrane-associated fatty acid photodecarboxylase sourced from Micractinium conductrix (McFAP). This endeavour resulted in creating an innovative assembled photoenzyme-membrane (protein load 5 mg cm ), subsequently integrated into an illuminated flow apparatus to achieve uninterrupted generation of alkane biofuels. Through batch experiments, the photoenzyme-membrane exhibited its prowess in converting fatty acids spanning varying chain lengths (C6-C18). Following this, the membrane-flow mesoscale reactor attained a maximum space-time yield of 1.2 mmol L h (C8) and demonstrated commendable catalytic proficiency across eight consecutive cycles, culminating in a cumulative runtime of eight hours. These findings collectively underscored the photoenzyme-membrane's capability to facilitate the biotransformation of diverse fatty acids, furnishing valuable benchmarks for the conversion of biomass via photobiocatalysis.
光生物催化烷烃生物燃料合成领域近年来蓬勃发展；然而，该领域目前缺乏成熟且可扩展的生产方法这一情况仍然很明显。在本研究中，我们对源自绿梭藻（Micractinium conductrix，简称McFAP）的膜相关脂肪酸光脱羧酶进行了改造。这一努力产生了一种创新的组装光酶 - 膜（蛋白质负载量为5 mg/cm），随后将其集成到光照流动装置中，以实现烷烃生物燃料的连续生成。通过批次实验，光酶 - 膜在转化不同链长（C6 - C18）的脂肪酸方面展现出了其能力。在此之后，膜流动中试规模反应器实现了1.2 mmol·L⁻¹·h⁻¹（C8）的最大时空产率，并在连续八个循环中表现出了出色的催化性能，累计运行时间达到了八小时。这些发现共同强调了光酶 - 膜促进多种脂肪酸生物转化的能力，为通过光生物催化转化生物质提供了有价值的基准。
