Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, PR China; Institute of Engineering Thermophysics, College of Energy and Power Engineering, Chongqing University, Chongqing 400044, PR China.
Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, PR China; Institute of Engineering Thermophysics, College of Energy and Power Engineering, Chongqing University, Chongqing 400044, PR China.
Bioresour Technol. 2023 Jan;367:128232. doi: 10.1016/j.biortech.2022.128232. Epub 2022 Nov 1.
As one of the fastest-growing carbon emission sources, the aviation sector is severely restricted by carbon emission reduction targets. Sustainable aviation fuel (SAF) has emerged as the most potential alternative to traditional aviation fuel, but harsh production technologies limit its commercialization. Fatty acids photodecarboxylase from Chlorella variabilis NC64A (CvFAP), the latest discovered photoenzyme, provides promising approaches to produce various carbon-neutral biofuels and fine chemicals. This review highlights the state-of-the-art strategies to enhance the application of CvFAP in carbon-neutral biofuel and fine chemicals production, including supplementing alkane as decoy molecular, screening efficient CvFAP variants with directed evolution, constructing genetic strains, employing biphasic catalytic system, and immobilizing CvFAP in an efficient photobioreactor. Furthermore, future opportunities are suggested to enhance photoenzymatic decarboxylation and explore the catalytic mechanism of CvFAP. This review provides a broad context to improve CvFAP catalysis and advance its potential applications.
作为增长最快的碳排放源之一，航空部门受到减排目标的严重限制。可持续航空燃料 (SAF) 已成为传统航空燃料最具潜力的替代品，但苛刻的生产技术限制了其商业化。来自变藻蓝菌 NC64A (CvFAP) 的脂肪酸脱羧酶是最新发现的光酶，为生产各种碳中和生物燃料和精细化学品提供了有前景的方法。本综述重点介绍了增强 CvFAP 在碳中和生物燃料和精细化学品生产中的应用的最新策略，包括补充烷烃作为诱饵分子、通过定向进化筛选高效的 CvFAP 变体、构建遗传菌株、采用双相催化系统和将 CvFAP 固定在高效光生物反应器中。此外，还提出了未来的机会，以增强光酶脱羧作用并探索 CvFAP 的催化机制。本综述提供了一个广泛的背景，以改善 CvFAP 催化并推进其潜在应用。
