Le Blancq S M, Peters W
Trans R Soc Trop Med Hyg. 1986;80(3):367-77. doi: 10.1016/0035-9203(86)90320-2.
Isoenzyme profiles of 67 stocks of Leishmania donovani sensu lato from across the Old World were compared with those of reference strains of L. donovani sensu stricto, L. infantum, L. major, L. tropica and L. aethiopica using starch-gel electrophoresis of 13 enzymes (GPI, GD, ES, PGM, PEPD, NH, ASAT, ALAT, PK, MPI, 6PGD, SOD, MDH). 12 zymodemes were seen. Isolates from man, Canis familiaris, Vulpes vulpes, Rattus rattus, Arvicanthis sp. and Phlebotomus martini were examined. Several zymodemes comprised stocks from man and C. familiaris, two of which also included wild animal isolates. Isolates from cases of post-kala-azar dermal leishmaniasis and visceral leishmaniasis were indistinguishable. L. donovani s.l., including L. donovani s.s. and L. infantum, formed a coherent group with a striking degree of enzymic homogeneity. Only one enzyme pattern was held in common with the L. tropica and L. aethiopica reference strains and two with the L. major reference strain enzyme profile.
利用13种酶（葡萄糖磷酸异构酶、谷氨酸脱氢酶、酯酶、磷酸葡萄糖变位酶、肽链内切酶、氨肽酶、天冬氨酸氨基转移酶、丙氨酸氨基转移酶、丙酮酸激酶、磷酸甘露糖异构酶、6-磷酸葡萄糖脱氢酶、超氧化物歧化酶、苹果酸脱氢酶）的淀粉凝胶电泳，将来自旧大陆各地的67株杜氏利什曼原虫复合种的同工酶谱与杜氏利什曼原虫指名亚种、婴儿利什曼原虫、硕大利什曼原虫、热带利什曼原虫和埃塞俄比亚利什曼原虫的参考菌株的同工酶谱进行了比较。共观察到12种酶带型。对来自人类、家犬、赤狐、褐家鼠、非洲刺毛鼠属和马丁白蛉的分离株进行了检测。几个酶带型包含来自人类和家犬的菌株，其中两个还包括野生动物分离株。来自黑热病后皮肤利什曼病和内脏利什曼病病例的分离株无法区分。杜氏利什曼原虫复合种，包括杜氏利什曼原虫指名亚种和婴儿利什曼原虫，形成了一个具有显著酶同质性的连贯群体。与热带利什曼原虫和埃塞俄比亚利什曼原虫参考菌株只有一种共同的酶谱型，与硕大利什曼原虫参考菌株的酶谱型有两种共同的酶谱型。
