Animal Genomics and Bioresource Research Unit (AGB Research Unit), Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Sciences for Industry, Faculty of Science, Kasetsart University, 50 Ngamwongwan, Chatuchak, Bangkok, 10900, Thailand.
Genes Genomics. 2024 Jan;46(1):113-119. doi: 10.1007/s13258-023-01459-6. Epub 2023 Nov 20.
The location of female-specific/linked loci identified in Siamese cobra (Naja kaouthia) previously has been determined through in silico chromosome mapping of the Indian cobra genome (N. naja) as a reference genome. In the present study, we used in silico chromosome mapping to identify sex-specific and linked loci in Siamese cobra. Many sex-specific and sex-linked loci were successfully mapped on the Z sex chromosome, with 227 of the 475 specific loci frequently mapped in a region covering 57 Mb and positioned at 38,992,675-95,561,177 bp of the Indian cobra genome (N. naja). This suggested the existence of a putative sex-determining region (SDR), with one specific locus (PA100000600) homologous to the TOPBP1 gene. The involvement of TOPBP1 gene may lead to abnormal synaptonemal complexes and meiotic chromosomal defects, resulting in male infertility. These findings offer valuable insights into the genetic basis and functional aspects of sex-specific traits in the Siamese cobra, which will contribute to our understanding of snake genetics and evolutionary biology.
先前，通过对印度眼镜蛇（Naja naja）基因组的计算机染色体作图，将暹罗眼镜蛇（Naja kaouthia）中的雌性/连锁基因座定位。在本研究中，我们使用计算机染色体作图来鉴定暹罗眼镜蛇的性别特异性和连锁基因座。许多性别特异性和性别连锁基因座成功地映射到 Z 性染色体上，其中 475 个特定基因座中有 227 个经常在一个覆盖 57 Mb 的区域中被映射，该区域位于印度眼镜蛇基因组（N. naja）的 38,992,675-95,561,177 bp 位置。这表明存在一个假定的性别决定区域（SDR），其中一个特定基因座（PA100000600）与 TOPBP1 基因同源。TOPBP1 基因的参与可能导致异常联会复合体和减数分裂染色体缺陷，导致男性不育。这些发现为暹罗眼镜蛇的性别特异性特征的遗传基础和功能方面提供了有价值的见解，将有助于我们理解蛇类遗传学和进化生物学。
