Whole-brain analysis of CO chemosensitive regions and identification of the retrotrapezoid and medullary raphé nuclei in the common marmoset ().

Respiratory chemosensitivity is an important mechanism by which the brain senses changes in blood partial pressure of CO (CO). It is proposed that special neurons (and astrocytes) in various brainstem regions play key roles as CO central respiratory chemosensors in rodents. Although common marmosets (), New-World non-human primates, show similar respiratory responses to elevated inspired CO as rodents, the chemosensitive regions in marmoset brain have not been defined yet. Here, we used c-fos immunostainings to identify brain-wide CO-activated brain regions in common marmosets. In addition, we mapped the location of the retrotrapezoid nucleus (RTN) and raphé nuclei in the marmoset brainstem based on colocalization of CO-induced c-fos immunoreactivity with Phox2b, and TPH immunostaining, respectively. Our data also indicated that, similar to rodents, marmoset RTN astrocytes express Phox2b and have complex processes that create a meshwork structure at the ventral surface of medulla. Our data highlight some cellular and structural regional similarities in brainstem of the common marmosets and rodents.