NEIKER - Basque Institute for Agricultural Research and Development, Derio, Spain.
Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU), Bilbao, Spain.
Bioessays. 2023 Dec;45(12):e2300153. doi: 10.1002/bies.202300153.
It is necessary to complement next-generation sequencing data on the soil resistome with theoretical knowledge provided by ecological studies regarding the spread of antibiotic resistant bacteria (ARB) in the abiotic and, especially, biotic fraction of the soil ecosystem. Particularly, when ARB enter agricultural soils as a consequence of the application of animal manure as fertilizer, from a microbial ecology perspective, it is important to know their fate along the soil food web, that is, throughout that complex network of feeding interactions among members of the soil biota that has crucial effects on species richness and ecosystem productivity and stability. It is critical to study how the ARB that enter the soil through the application of manure can reach other taxonomical groups (e.g., fungi, protists, nematodes, arthropods, earthworms), paying special attention to their presence in the gut microbiomes of mesofauna-macrofauna and to the possibilities for horizontal gene transfer of antibiotic resistant genes.
有必要将土壤抗药性组的下一代测序数据与生态研究提供的理论知识相结合，这些知识涉及抗生素耐药菌 (ARB) 在土壤生态系统的非生物和特别是生物部分中的传播。特别是，当 ARB 作为动物粪便作为肥料施用于农业土壤时，从微生物生态学的角度来看，了解它们在土壤食物网中的命运非常重要，也就是说，了解它们在土壤生物群落成员之间复杂的食物链中如何发挥作用，这对物种丰富度和生态系统生产力和稳定性具有至关重要的影响。研究通过施用粪便进入土壤的 ARB 如何能够到达其他分类群（例如真菌、原生动物、线虫、节肢动物、蚯蚓）非常重要，特别要注意它们在中大型土壤动物肠道微生物组中的存在，以及抗生素耐药基因发生水平基因转移的可能性。
