Silva Ana P B, Roque-Borda Cesar Augusto, Carnero Canales Christian S, Duran Gleriani Primo Laura Maria, Silva Isabel C, Ribeiro Camila M, Chorilli Marlus, da Silva Patrícia Bento, Silva Joás L, Pavan Fernando Rogério
Tuberculosis Research Laboratory, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil.
Facultad de Ciencias Farmaceuticas, Bioquímicas y Biotecnológicas, Universidad Católica de Santa María, Arequipa 04000, Peru.
Diseases. 2023 Oct 26;11(4):150. doi: 10.3390/diseases11040150.
The search for new antimicrobial agents is a continuous struggle, mainly because more and more cases of resistant strains are being reported. (MTB) is the main microorganism responsible for millions of deaths worldwide. The development of new antimicrobial agents is generally aimed at finding strong interactions with one or more bacterial receptors. It has been proven that bacteriophages have the ability to adhere to specific and selective regions. However, their transport and administration must be carefully evaluated as an excess could prevent a positive response and the bacteriophages may be eliminated during their journey. With this in mind, the mycobacteriophage D29 was encapsulated in nanoliposomes, which made it possible to determine its antimicrobial activity during transport and its stability in the treatment of active and latent . The antimicrobial activity, the cytotoxicity in macrophages and fibroblasts, as well as their infection and time-kill were evaluated. Phage nanoencapsulation showed efficient cell internalization to induce MTB clearance with values greater than 90%. Therefore, it was shown that nanotechnology is capable of assisting in the activity of degradation-sensitive compounds to achieve better therapy and evade the immune response against phages during treatment.
寻找新型抗菌剂是一场持续的斗争，主要是因为耐药菌株的病例报告越来越多。结核分枝杆菌（MTB）是导致全球数百万人死亡的主要微生物。新型抗菌剂的开发通常旨在寻找与一种或多种细菌受体的强相互作用。已经证明噬菌体能够附着在特定的选择性区域。然而，必须仔细评估它们的运输和给药，因为过量可能会阻止阳性反应，并且噬菌体可能在其旅程中被清除。考虑到这一点，分枝杆菌噬菌体D29被包裹在纳米脂质体中，这使得在运输过程中确定其抗菌活性以及在治疗活动性和潜伏性疾病时的稳定性成为可能。评估了抗菌活性、对巨噬细胞和成纤维细胞的细胞毒性以及它们的感染和杀菌时间。噬菌体纳米封装显示出高效的细胞内化，能够诱导MTB清除率超过90%。因此，研究表明纳米技术能够辅助降解敏感化合物的活性，以实现更好的治疗，并在治疗过程中规避针对噬菌体的免疫反应。
