Abyadeh Morteza, Gupta Vivek, Liu Xinyue, Rossio Valentina, Mirzaei Mehdi, Cornish Jennifer, Paulo Joao A, Haynes Paul A
ProGene Technologies Pty Ltd., Macquarie Park, NSW 2113, Australia.
Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, Australia.
Proteomes. 2023 Nov 2;11(4):36. doi: 10.3390/proteomes11040036.
Cannabis has been used historically for both medicinal and recreational purposes, with the most notable cannabinoids being cannabidiol (CBD) and tetrahydrocannabinol (THC). Although their therapeutic effects have been well studied and their recreational use is highly debated, the underlying mechanisms of their biological effects remain poorly defined. In this study, we use isobaric tag-based sample multiplexed proteome profiling to investigate protein abundance differences in the human neuroblastoma SH-SY5Y cell line treated with CBD and THC. We identified significantly regulated proteins by each treatment and performed a pathway classification and associated protein-protein interaction analysis. Our findings suggest that these treatments may lead to mitochondrial dysfunction and induce endoplasmic reticulum stress. These data can potentially be interrogated further to investigate the potential role of CBD and THC in various biological and disease contexts, providing a foundation for future studies.
大麻在历史上一直被用于医疗和娱乐目的，最著名的大麻素是大麻二酚（CBD）和四氢大麻酚（THC）。尽管它们的治疗效果已得到充分研究，且其娱乐用途备受争议，但其生物学效应的潜在机制仍不清楚。在本研究中，我们使用基于等压标签的样本多重蛋白质组分析来研究用CBD和THC处理的人神经母细胞瘤SH-SY5Y细胞系中的蛋白质丰度差异。我们确定了每种处理下显著调节的蛋白质，并进行了通路分类和相关的蛋白质-蛋白质相互作用分析。我们的研究结果表明，这些处理可能导致线粒体功能障碍并诱导内质网应激。这些数据有可能进一步深入研究，以探究CBD和THC在各种生物学和疾病背景中的潜在作用，为未来的研究提供基础。
