Hua Zhongjie, Zhang Jiachan, Cheng Wenjing, Wang Changtao, Zhao Dan
Beijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China.
Institute of Cosmetic Regulatory Science, Beijing Technology and Business University, Beijing 100048, China.
Foods. 2023 Sep 4;12(17):3322. doi: 10.3390/foods12173322.
L. has been widely used in research and application for almost two decades. While significant progress was achieved in the examination of its fruits and seeds, the exploration and utilization of its by-products have received relatively less attention. This study aims to address this research gap by investigating the effects and underlying mechanisms of sea buckthorn seed residues both in vitro and in vivo. The primary objective of this study is to assess the potential of the hydroalcoholic extract from sea buckthorn seed residues (HYD-SBSR) to prevent cell apoptosis and mitigate oxidative stress damage. To achieve this, an HO-induced B16F10 cell model and a D-galactose-induced mouse model were used. The HO-induced oxidative stress model using B16F10 cells was utilized to evaluate the cellular protective and reparative effects of HYD-SBSR. The results demonstrated the cytoprotective effects of HYD-SBSR, as evidenced by reduced apoptosis rates and enhanced resistance to oxidative stress alongside moderate cell repair properties. Furthermore, this study investigated the impact of HYD-SBSR on antioxidant enzymes and peroxides in mice to elucidate its reparative potential in vivo. The findings revealed that HYD-SBSR exhibited remarkable antioxidant performance, particularly at low concentrations, significantly enhancing antioxidant capacity under oxidative stress conditions. To delve into the mechanisms underlying HYD-SBSR, a comprehensive proteomics analysis was conducted to identify differentially expressed proteins (DEPs). Additionally, a Gene Ontology (GO) analysis and an Encyclopedia of Genes and Genomes (KEGG) pathway cluster analysis were performed to elucidate the functional roles of these DEPs. The outcomes highlighted crucial mechanistic pathways associated with HYD-SBSR, including the PPAR signaling pathway, fat digestion and absorption, glycerophospholipid metabolism, and cholesterol metabolism. The research findings indicated that HYD-SBSR, as a health food supplement, exhibits favorable effects by promoting healthy lipid metabolism, contributing to the sustainable and environmentally friendly production of sea buckthorn and paving the way for future investigations and applications in the field of nutraceutical and pharmaceutical research.
近二十年来，L.已广泛应用于研究和实际应用中。虽然在其果实和种子的研究方面取得了显著进展，但其副产品的开发利用却相对较少受到关注。本研究旨在通过研究沙棘籽渣在体外和体内的作用及其潜在机制来填补这一研究空白。本研究的主要目的是评估沙棘籽渣水醇提取物（HYD-SBSR）预防细胞凋亡和减轻氧化应激损伤的潜力。为此，使用了过氧化氢诱导的B16F10细胞模型和D-半乳糖诱导的小鼠模型。利用B16F10细胞的过氧化氢诱导氧化应激模型来评估HYD-SBSR的细胞保护和修复作用。结果表明HYD-SBSR具有细胞保护作用，表现为凋亡率降低、抗氧化应激能力增强以及适度的细胞修复特性。此外，本研究还考察了HYD-SBSR对小鼠抗氧化酶和过氧化物的影响，以阐明其在体内的修复潜力。研究结果表明，HYD-SBSR具有显著的抗氧化性能，尤其是在低浓度时，能在氧化应激条件下显著提高抗氧化能力。为深入探究HYD-SBSR的潜在机制，进行了全面的蛋白质组学分析以鉴定差异表达蛋白（DEP）。此外，还进行了基因本体（GO）分析和基因与基因组百科全书（KEGG）通路聚类分析，以阐明这些DEP的功能作用。结果突出了与HYD-SBSR相关的关键机制途径，包括过氧化物酶体增殖物激活受体（PPAR）信号通路、脂肪消化与吸收、甘油磷脂代谢和胆固醇代谢。研究结果表明，HYD-SBSR作为一种健康食品补充剂，通过促进健康的脂质代谢发挥有益作用，有助于沙棘的可持续和环境友好型生产，并为营养保健品和药物研究领域的未来研究及应用铺平道路。
