Institut Pasteur, Université Paris Cité, CNRS UMR3523, Department of Structural Biology and Chemistry, Laboratory for Bioorganic Chemistry of Nucleic Acids, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France.
Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, Grenzacherstrasse 124, 4070, Basel, Switzerland.
Chem Commun (Camb). 2023 Dec 7;59(98):14547-14550. doi: 10.1039/d3cc04933g.
Herein, we report the high-yielding solid-phase synthesis of unmodified and chemically modified trinucleotide triphosphates (dNTPs). These synthetic codons can be used for enzymatic DNA synthesis provided their scaffold is stabilized with phosphorothioate units. Enzymatic synthesis with three rather than one letter nucleotides will be useful to produce xenonucleic acids (XNAs) and for selection of modified functional nucleic acids.
在此，我们报告了未修饰和化学修饰的三核苷酸三磷酸（dNTP）的高产固相合成。这些合成密码子可以与硫代磷酸酯单元稳定其支架，用于酶促 DNA 合成。使用三个而不是一个字母核苷酸的酶促合成将有助于产生异核酸（XNA）和选择修饰的功能性核酸。
