Department of Surgery, Yale School of Medicine, Yale University, New Haven, CT.
Department of Medicine, Yale Cancer Center, Yale University, New Haven, CT.
J Immunother. 2024;47(2):49-53. doi: 10.1097/CJI.0000000000000495. Epub 2023 Nov 22.
Adoptive transfer of ex vivo expanded tumor-infiltrating lymphocytes (TILs) have produced long-term response in metastatic cancers. TILs have traditionally been expanded from surgically resected specimens. Ultrasound-guided core needle biopsy (CNB) is an alternative method that avoids the morbidity of surgery and have added benefits which may include patients not amenable to surgery as well as the potential to produce TILs from multiple lesions in the same patient. We assessed the ability to produce and expand TILs from primary triple-negative breast cancer tumors from CNB (n=7) and demonstrate comparable expansion, phenotype and cytokine secretion after phorbol myristate acetate-ionomycin stimulation to TILs expanded from surgery (n=6). T cell Receptor clonality and diversity were also comparable between the two cohorts throughout the TIL culture. CNB is a safe and feasible method to obtain tumor tissue for TIL generation in patients with triple-negative breast cancer.
过继输注体外扩增的肿瘤浸润淋巴细胞（TIL）已在转移性癌症中产生了长期反应。TIL 传统上是从手术切除的标本中扩增而来。超声引导下的核心针活检（CNB）是一种替代方法，可避免手术带来的发病率，并且具有一些额外的益处，包括那些不适宜手术的患者，以及有可能从同一患者的多个病变部位产生 TIL。我们评估了从 CNB 获得的原发性三阴性乳腺癌肿瘤中产生和扩增 TIL 的能力（n=7），并证明了与从手术中扩增的 TIL（n=6）相比，在佛波醇肉豆蔻酸乙酸酯-离子霉素刺激后具有相当的扩增、表型和细胞因子分泌能力。在整个 TIL 培养过程中，两组的 T 细胞受体克隆性和多样性也相似。CNB 是一种安全且可行的方法，可从三阴性乳腺癌患者中获得肿瘤组织以用于 TIL 生成。
