Department of Pediatrics, Division of Pulmonary, Asthma, Cystic Fibrosis, and Sleep, Emory University School of Medicine, Atlanta, Georgia, USA.
Emory+Children's Center for Cystic Fibrosis and Airway Disease Research, Emory University School of Medicine, Atlanta, Georgia, USA.
Infect Immun. 2023 Dec 12;91(12):e0024723. doi: 10.1128/iai.00247-23. Epub 2023 Nov 22.
There are currently no approved vaccines against the opportunistic pathogen . Among vaccine targets, the lipopolysaccharide (LPS) O antigen of is the most immunodominant protective candidate. There are 20 different O antigens composed of different repeat sugar structures conferring serogroup specificity, and 10 are found most frequently in infection. Thus, one approach to combat infection by could be to generate immunity with a vaccine cocktail that includes all these serogroups. Serogroup O9 is 1 of the 10 serogroups commonly found in infection, but it has never been developed into a vaccine, due in part to the acid-labile nature of the O9 polysaccharide. Our laboratory has previously shown that intranasal administration of an attenuated strain expressing the serogroup O11 LPS O antigen was effective in clearing bacteria and preventing mortality in mice following intranasal challenge with serogroup O11 . Consequently, we set out to develop a serogroup O9 vaccine using a similar approach. Here, we show that expressing serogroup O9 triggered an antibody-mediated immune response following intranasal administration to mice and that it conferred protection from serogroup O9 in a murine model of acute pneumonia.
目前尚无针对机会性病原体的批准疫苗。在疫苗靶标中，的脂多糖（LPS）O 抗原是最具免疫优势的保护性候选物。有 20 种不同的 O 抗原由赋予血清组特异性的不同重复糖结构组成，其中 10 种在感染中最常发现。因此，对抗感染的一种方法可能是使用包含所有这些血清组的疫苗鸡尾酒来产生免疫力。血清组 O9 是感染中最常发现的 10 个血清组之一，但由于 O9 多糖的酸不稳定性质，从未将其开发成疫苗。我们的实验室之前曾表明，鼻腔内给予表达血清组 O11 LPS O 抗原的减毒 株可有效清除细菌，并在鼻腔内用血清组 O11 攻击后预防小鼠的死亡率。因此，我们着手使用类似的方法开发血清组 O9 疫苗。在这里，我们表明，鼻腔内给予表达血清组 O9 的可引发抗体介导的免疫反应，并且在急性肺炎的小鼠模型中可提供针对血清组 O9 的保护。
