Laboratorio NEST, Scuola Normale Superiore, Pisa, Italy.
Chiesi Farmaceutici S.p.A., Parma, Italy.
Nanoscale. 2023 Dec 7;15(47):19085-19090. doi: 10.1039/d3nr03420h.
Here we use fluorescence lifetime imaging microscopy (FLIM) to study the supramolecular organization of nanoencapsulated liposomal all- retinoic acid (ATRA), exploiting ATRA's intrinsic fluorescence as a source of signal and phasor transformation as a fit-free analytical approach to lifetime data. Our non-invasive method is suitable for checking for the presence of a fraction of ATRA molecules interacting with liposomal membranes. The results are validated by independent small-angle X-ray scattering (SAXS) and nano-differential scanning calorimetry (NanoDSC) measurements, probing ATRA's putative position on the membrane and effect on membrane organization. Besides the insights on the specific case-study proposed, the present results confirm the effectiveness of Phasor-FLIM analysis in elucidating the nanoscale supramolecular organization of fluorescent drugs in pharmaceutical formulations. This underscores the importance of leveraging advanced imaging techniques to deepen our understanding and optimize drugs' performance in delivery applications.
在这里，我们使用荧光寿命成像显微镜（FLIM）来研究纳米封装脂质体全反式视黄酸（ATRA）的超分子结构，利用 ATRA 的固有荧光作为信号源，并采用相向量变换作为无拟合分析方法来处理寿命数据。我们的非侵入性方法适用于检查与脂质体膜相互作用的 ATRA 分子部分的存在情况。通过独立的小角 X 射线散射（SAXS）和纳米差示扫描量热法（NanoDSC）测量对结果进行了验证，这些测量方法探测了 ATRA 在膜上的可能位置及其对膜组织的影响。除了对所提出的具体案例研究的深入了解外，本研究结果还证实了相向量-FLIM 分析在阐明药物制剂中荧光药物的纳米级超分子结构方面的有效性。这突显了利用先进的成像技术来加深我们对药物在输送应用中的性能的理解和优化的重要性。
