School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Library, Wuhan University of Technology, Wuhan 430070, China.
Sci Total Environ. 2024 Feb 1;910:168737. doi: 10.1016/j.scitotenv.2023.168737. Epub 2023 Nov 20.
Niclosamide (NIC) is a commonly used molluscicide that reportedly disrupts the endocrine system and may lead to lipid metabolism disorders. However, few studies have investigated the mechanism by which NIC affects the endocrine system from the perspective of lipid metabolism. Adult female zebrafish were fed either a normal-fat diet (NFD) or a high-fat diet (HFD) and then exposed for 28 days to environmentally relevant concentrations of NIC. NIC accumulated most in the liver followed by the brain and then the gonads in both feeding conditions. Somatic index changes confirmed that HFD promotes fish growth, and NIC administration inhibits it. Lipid metabolites were decreased by NIC, as were levels of pregnenolone, androstenedione, estrogen, testosterone, and estradiol, suggesting that NIC impacted steroidogenesis. In addition, gene transcription changes related to the hypothalamic-pituitary-gonad-liver (HPGL) axis and altered ovarian histology strongly suggest that environmental relevant concentrations of NIC exposure may disrupt endocrine function. These findings highlighted that NIC exposure at environmentally relevant concentrations elicited endocrine-disruption effects may through impairing of steroid hormone synthesis.
硝氯酚（NIC）是一种常用的灭螺剂，据报道它会破坏内分泌系统，并可能导致脂质代谢紊乱。然而，很少有研究从脂质代谢的角度探讨 NIC 影响内分泌系统的机制。将成年雌性斑马鱼分别用正常脂肪饮食（NFD）或高脂肪饮食（HFD）喂养，然后用环境相关浓度的 NIC 暴露 28 天。在两种喂养条件下，NIC 在肝脏中积累最多，其次是大脑，然后是性腺。体指数变化证实 HFD 促进鱼类生长，而 NIC 给药则抑制生长。NIC 降低了脂质代谢物、孕烯醇酮、雄烯二酮、雌激素、睾酮和雌二醇的水平，表明 NIC 影响了类固醇生成。此外，与下丘脑-垂体-性腺-肝（HPGL）轴相关的基因转录变化和卵巢组织学改变强烈表明，环境相关浓度的 NIC 暴露可能会破坏内分泌功能。这些发现强调了环境相关浓度的 NIC 暴露可能通过损害类固醇激素合成来引发内分泌干扰效应。
