College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China; School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, China; Suzhou Qinpu Biotechnology Pte Ltd, Suzhou, Jiangsu Province 215215, China.
College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry of Agriculture, Shanghai 201306, China; National R & D Branch Center for Freshwater Aquatic Products Processing Technology, Shanghai 201306, China.
Int J Pharm. 2024 Jan 25;650:123629. doi: 10.1016/j.ijpharm.2023.123629. Epub 2023 Nov 20.
Given that current androgenetic alopecia (AGA) medications have adverse effects such as sexual dysfunction and drug dependence, researchers are actively exploring natural bioactive ingredients and innovative approaches (e.g., transdermal drug delivery systems) to effectively combat hair loss with minimal side effects. Herein, we develop a new transdermal drug delivery system incorporating globefish skin collagen peptides with dissolving microneedles (GSCPs-MNs) for hair regrowth. These microneedles generate skin micro-wounds upon application, which not only improves the efficiency of bioactive ingredients delivery, but also stimulates signals involved in hair follicle (HF) regeneration. Our in vivo study shows that minimally invasive implanted GSCPs-MNs are more effective than topical GSCPs in reducing inflammation and promoting collagen formation. Additionally, the upregulation of vascular markers including VEGF and CD31 alongside the downregulation of TNF-α, IL-1β, and malondialdehyde (MDA) index indicate that GSCPs-MNs can significantly alleviate inflammation and oxidation, as well as promoting vascularization and HF functionalization. Overall, our findings suggest that GSCPs-MNs can effectively promote hair regrowth in AGA mice, which offer excellent prospects for the development of new therapeutics and cosmetic supplements for hair loss, along with the combined drug delivery optimization, which could alleviate hair loss in patients with AGA.
鉴于目前的雄激素性脱发 (AGA) 药物具有性功能障碍和药物依赖等不良反应，研究人员正在积极探索天然生物活性成分和创新方法（例如，透皮药物递送系统），以最小的副作用有效治疗脱发。在此，我们开发了一种新的透皮药物递送系统，该系统将绒鱼皮胶原蛋白肽与可溶解微针 (GSCPs-MNs) 结合，用于头发生长。这些微针在应用时会产生皮肤微创伤，不仅提高了生物活性成分的递送效率，还刺激了参与毛囊 (HF) 再生的信号。我们的体内研究表明，微创植入的 GSCPs-MNs 比局部 GSCPs 更有效地减少炎症和促进胶原蛋白形成。此外，血管标志物（包括 VEGF 和 CD31）的上调以及 TNF-α、IL-1β 和丙二醛 (MDA) 指数的下调表明，GSCPs-MNs 可以显著减轻炎症和氧化，促进血管生成和 HF 功能化。总体而言，我们的研究结果表明，GSCPs-MNs 可以有效促进 AGA 小鼠的头发生长，为脱发的新型治疗药物和化妆品补充剂的开发提供了广阔的前景，同时也为联合药物递送优化提供了可能，从而减轻 AGA 患者的脱发问题。
