National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, East China University of Science and Technology, Shanghai, China; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, China.
National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, East China University of Science and Technology, Shanghai, China; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, China.
Environ Res. 2024 Feb 15;243:117738. doi: 10.1016/j.envres.2023.117738. Epub 2023 Nov 20.
Algae have great application prospects in excess sludge reclamation and recovery of high-value biomass. Chlorococcum humicola was cultivated in this research, using sludge extract (mixed with SE medium) with additions of 10%, 20%, and 30% CO (v/v). Results showed that under 20% CO, the dry weight and polysaccharide yield reached 1.389 ± 0.070 g/L and 313.49 ± 10.77 mg/L, respectively. 10% and 20% CO promoted the production of cellular antioxidant molecules to resist the toxic stress and the toxicity of 20% CO group decreased from 62.16 ± 3.11% to 33.02 ± 3.76%. 10% and 20% CO accelerated the electron transfer, enhanced carbon assimilation, and promoted the photosynthetic efficiency, while 30% CO led to photosystem damage and disorder of antioxidant system. Proteomic analysis showed that 20% CO mainly affected energy metabolism and the oxidative stress level on the early stage (10 d), while affected photosynthesis and organic substance metabolism on the stable stage (30 d). The up-regulation of PSII photosynthetic protein subunit 8 (PsbA, PsbO), A0A383W1S5 and A0A383VRI4 promoted the efficiency of PSII and chlorophyll synthesis, and the up-regulation of A0A383WH74 and A0A2Z4THB7 led to the accumulation of polysaccharides. The up-regulation of A0A383VDH1, A0A383VX37 and A0A383VA86 promoted respiration. Collectively, this work discloses the regulatory mechanism of high-concentration CO on Chlorococcum humicola to overcome toxicity and accumulate polysaccharides.
藻类在剩余污泥的回收和高价值生物质的回收方面具有广阔的应用前景。本研究以污泥提取物（与 SE 培养基混合）为基础，添加 10%、20%和 30%（v/v）的 CO 培养集胞小球藻。结果表明，在 20% CO 条件下，干重和多糖产量分别达到 1.389±0.070 g/L 和 313.49±10.77 mg/L。10%和 20% CO 促进了细胞抗氧化分子的产生，以抵抗有毒胁迫，20% CO 组的毒性从 62.16±3.11%降低到 33.02±3.76%。10%和 20% CO 加速了电子传递，增强了碳同化，提高了光合作用效率，而 30% CO 导致光系统损伤和抗氧化系统紊乱。蛋白质组学分析表明，20% CO 主要在早期（10 d）影响能量代谢和氧化应激水平，而在稳定期（30 d）影响光合作用和有机物质代谢。PSII 光合作用蛋白亚基 8（PsbA、PsbO）、A0A383W1S5 和 A0A383VRI4 的上调促进了 PSII 和叶绿素的合成效率，A0A383WH74 和 A0A2Z4THB7 的上调导致多糖的积累。A0A383VDH1、A0A383VX37 和 A0A383VA86 的上调促进了呼吸作用。综上所述，本研究揭示了高浓度 CO 对集胞小球藻克服毒性和积累多糖的调控机制。
