Chen Xiurong, Ding Biao, Zhang Xinyu, Yu Jiayu, Song Meijing, Li Renjie
National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China.
State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control On Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Environ Sci Pollut Res Int. 2023 May;30(22):62867-62879. doi: 10.1007/s11356-023-25554-z. Epub 2023 Mar 22.
Microalgae such as Tetradesmus obliquus have great potential in immobilizing high-concentration CO and removing highly toxic organic matters, which could be produced from coal chemical industry and coal chemical wastewater biological treatment process. In this study, Tetradesmus obliquus was cultured in sludge extract and high-concentration CO was added. The maximum cell density and dry weight were respectively (111.46 ± 4.87) × 10 cell/mL and 3.365 ± 0.168 g/L under 30% CO. Tetradesmus obliquus accumulated the most polysaccharides (629.60 ± 31.48 mg/L) on the 30th day under 30% CO. The results of proteomic showed that the upregulation of A0A2Z4THB7 and A0A383VAT1 promoted polysaccharide accumulation. Polysaccharide was mainly formed at the stable phase instead of the log-growth phase due to the abiotic stress caused by high TOC at the log-growth phase. Collectively, this study revealed the regulatory mechanism of high-concentration CO on the toxicity removal and accumulation of polysaccharides in Tetradesmus obliquus.
斜生栅藻等微藻在固定高浓度一氧化碳和去除高毒性有机物方面具有巨大潜力，这些物质可能来自煤化工及煤化工废水生物处理过程。本研究中，斜生栅藻在污泥提取物中培养并添加高浓度一氧化碳。在30%一氧化碳条件下，最大细胞密度和干重分别为(111.46 ± 4.87)×10⁶个细胞/毫升和3.365 ± 0.168克/升。在30%一氧化碳条件下，斜生栅藻在第30天积累的多糖最多(629.60 ± 31.48毫克/升)。蛋白质组学结果表明，A0A2Z4THB7和A0A383VAT1的上调促进了多糖积累。由于对数生长期高总有机碳引起的非生物胁迫，多糖主要在稳定期而非对数生长期形成。总体而言，本研究揭示了高浓度一氧化碳对斜生栅藻毒性去除和多糖积累的调控机制。
