Inje University College of Medicine, Busan, Republic of Korea.
Department of Clinical Laboratory Science, Semyung University, Jecheon, Republic of Korea.
Anaerobe. 2023 Dec;84:102804. doi: 10.1016/j.anaerobe.2023.102804. Epub 2023 Nov 20.
The purpose of this study is to evaluate the usefulness of anaerobic blood culture in pediatric patients by comparing the detection rate and distribution of bacteria between aerobic and anaerobic blood culture bottles.
A retrospective analysis was conducted on 11,664 blood cultures obtained from children under the age of 14 between January 2013 and June 2020. The positive rate of total, aerobic, and anaerobic blood culture, as well as the species distribution of each blood culture bottle, were investigated.
The positive rate of blood culture was 2.4 % (N = 281). Among them, 67 (23.8 %), 85 (30.3 %) and 129 (45.9 %) organisms were grown in only aerobic, only anaerobic, and both blood culture bottles, respectively. Gram-positive cocci were cultured on both, only aerobic, and only anaerobic blood culture bottles in proportions of 46.4 %, 23.4 %, and 30.2 %, respectively. Gram-negative bacilli were cultured on both, only aerobic, and only anaerobic blood culture bottles in proportions of 58.5 %, 12,3 %, and 29.2 %, respectively. Gram-positive bacilli grew best in aerobic bottle only. There were seven strains of obligate anaerobes.
Because many facultative anaerobic bacteria are recognized primarily from anaerobic blood culture bottles, combining aerobic and anaerobic blood culture bottles might be beneficial in pediatric patients with suspected blood stream infection.
本研究旨在通过比较需氧和厌氧血培养瓶中细菌的检出率和分布，评估厌氧血培养在儿科患者中的应用价值。
对 2013 年 1 月至 2020 年 6 月期间 14 岁以下儿童的 11664 份血培养进行回顾性分析。调查总血培养、需氧血培养和厌氧血培养的阳性率，以及每个血培养瓶的菌种分布。
血培养阳性率为 2.4%（N=281）。其中，仅需氧、仅厌氧和两种血培养瓶中分别培养出 67（23.8%）、85（30.3%）和 129（45.9%）种微生物。两种血培养瓶均培养出革兰阳性球菌，分别占 46.4%、23.4%和 30.2%；两种血培养瓶均培养出革兰阴性杆菌，分别占 58.5%、12.3%和 29.2%；仅需氧血培养瓶培养出革兰阳性杆菌，占比 30.2%。共培养出 7 株严格厌氧菌。
由于许多兼性厌氧菌主要从厌氧血培养瓶中检出，因此对疑似血流感染的儿科患者联合进行需氧和厌氧血培养可能有益。
