Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Central South Academy of Inventory and Planning of NFGA, Changsha, 410014, China.
Sci Data. 2023 Nov 22;10(1):819. doi: 10.1038/s41597-023-02742-7.
The application of DNA barcoding has been significantly limited by the scarcity of reliable specimens and inadequate coverage and replication across all species. The deficiency of DNA barcode reference coverage is particularly striking for highly biodiverse subtropical and tropical regions. In this study, we present a comprehensive barcode library for woody plants in tropical and subtropical China. Our dataset includes a standard barcode library comprising the four most widely used barcodes (rbcL, matK, ITS, and ITS2) for 2,520 species from 4,654 samples across 49 orders, 144 families, and 693 genera, along with 79 samples identified at the genus level. This dataset also provides a super-barcode library consisting of 1,239 samples from 1,139 species, 411 genera, 113 families, and 40 orders. This newly developed library will serve as a valuable resource for DNA barcoding research in tropical and subtropical China and bordering countries, enable more accurate species identification, and contribute to the conservation and management of tropical and subtropical forests.
DNA 条形码的应用受到可靠标本稀缺、所有物种的覆盖范围和复制不足的显著限制。对于生物多样性极高的亚热带和热带地区，DNA 条形码参考覆盖范围的不足尤为明显。在这项研究中，我们为中国热带和亚热带的木本植物提供了一个全面的条形码文库。我们的数据集包括一个标准的条形码文库，包含了来自 49 个目、144 个科和 693 个属的 4654 个样本中的 2520 个物种的四个最广泛使用的条形码（rbcL、matK、ITS 和 ITS2），以及 79 个在属水平上鉴定的样本。该数据集还提供了一个超级条形码文库，包含了来自 1139 个物种、411 个属、113 个科和 40 个目的 1239 个样本。这个新开发的文库将成为中国热带和亚热带及周边国家 DNA 条形码研究的宝贵资源，能够实现更准确的物种鉴定，并有助于热带和亚热带森林的保护和管理。
