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长疣蛛毒液腺转录组学和毒液蛋白质组学研究。

Venom-gland transcriptomics and venom proteomics of the Tibellus oblongus spider.

机构信息

Department of Molecular Neurobiology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 16/10 Miklukho-Maklay Str., 117997, Moscow, Russia.

Scientific Research Institute for Systems Biology and Medicine, Scientific Driveway, 18, 117246, Moscow, Russia.

出版信息

Sci Data. 2023 Nov 22;10(1):820. doi: 10.1038/s41597-023-02703-0.

DOI:10.1038/s41597-023-02703-0
PMID:37993463
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10665394/
Abstract

The Tibellus oblongus spider is an active hunter that does not spin webs and remains highly underinvestigated in terms of the venom composition. Here, we describe venom glands transcriptome and venom proteome analysis for unveiling the polypeptide composition of Tibellus oblongus spider venom. The resulting EST database includes 1733 records, including 1263 nucleotide sequences with ORFs, of these 942 have been identified as toxin-coding. The database of peptide sequences was built based on of the transcriptomics results. It contains 217 new toxins, 212 of them were detected in the T. oblongus venom by the proteomics.

摘要

狭长长足蛛是一种活跃的猎手，它不结网，而且其毒液成分的研究还很不充分。在这里，我们描述了毒液腺转录组和毒液蛋白质组分析，以揭示狭长长足蛛毒液的多肽组成。由此产生的 EST 数据库包括 1733 条记录，其中包括 1263 条带有 ORF 的核苷酸序列，其中 942 条已被鉴定为毒素编码。肽序列数据库是基于转录组学结果构建的。它包含 217 种新毒素，其中 212 种在 T. oblongus 毒液中通过蛋白质组学检测到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/6c67ffc2d30f/41597_2023_2703_Fig13_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/6c67ffc2d30f/41597_2023_2703_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/8432b2d024b2/41597_2023_2703_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/7f4a9b88e767/41597_2023_2703_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/20647c127805/41597_2023_2703_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/2b2868331e33/41597_2023_2703_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/79212ff63158/41597_2023_2703_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/55a8c1b8a21b/41597_2023_2703_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/66bf47ba9ce2/41597_2023_2703_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/14239779e2ba/41597_2023_2703_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/c84a8811bfe6/41597_2023_2703_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/8e44fd9e232d/41597_2023_2703_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/c3c5813b73c3/41597_2023_2703_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/22a37d6c6607/41597_2023_2703_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3f/10665394/6c67ffc2d30f/41597_2023_2703_Fig13_HTML.jpg

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