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我们的做法:苏黎世显微外科实验室胎盘准备技术。

How we do it: the Zurich Microsurgery Lab technique for placenta preparation.

机构信息

Zurich Microsurgery Lab, Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, University of Zurich, Frauenklinikstrasse 10, 8091, Zurich, Switzerland.

出版信息

Acta Neurochir (Wien). 2023 Dec;165(12):3821-3824. doi: 10.1007/s00701-023-05847-5. Epub 2023 Nov 23.

DOI:10.1007/s00701-023-05847-5
PMID:37993631
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10739554/
Abstract

BACKGROUND

Perfused placentas provide an excellent and accessible model for microvascular dissection, microsuturing and microanastomosis training - particularly in the early microsurgical learning curve. This way, a significant amount of live animals can be spared.

METHOD

We present the Zurich Microsurgery Lab protocol, detailing steps for obtaining, selecting, cleaning, flushing, cannulating, and preserving human placentas - as well as microsurgical training examples - in a tried-and-true, safe, cost-effective, and high-yield fashion.

CONCLUSION

Our technique enables highly realistic microsurgical training (microdissection, microvascular repair, microanastomosis) based on readily available materials. Proper handling, preparation, and preservation of the perfused placenta models is key.

摘要

背景

灌流胎盘为微血管解剖、显微缝合和微血管吻合训练提供了一个极好且易于获取的模型,尤其是在早期的显微外科学习曲线中。这样,可以节省大量的活体动物。

方法

我们介绍了苏黎世显微外科实验室的方案,详细说明了获取、选择、清洁、冲洗、插管和保存人胎盘的步骤,以及在经过验证的、安全、具有成本效益和高产的方式下进行显微外科训练的示例。

结论

我们的技术基于现成的材料,实现了高度逼真的显微外科训练(显微解剖、血管修复、吻合术)。适当的处理、准备和保存灌流胎盘模型是关键。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c528/10739554/54b11a2479f6/701_2023_5847_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c528/10739554/e7d4cd22af5b/701_2023_5847_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c528/10739554/54b11a2479f6/701_2023_5847_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c528/10739554/e7d4cd22af5b/701_2023_5847_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c528/10739554/54b11a2479f6/701_2023_5847_Fig2_HTML.jpg

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本文引用的文献

1
Dye-Perfused Human Placenta for Vascular Microneurosurgery Training: Preparation Protocol and Validation Testing.染料灌注人胎盘用于血管微神经外科培训:准备方案和验证测试。
World Neurosurg. 2021 Feb;146:e854-e864. doi: 10.1016/j.wneu.2020.11.034. Epub 2020 Nov 14.
2
Human Placenta Simulator for Intracranial-Intracranial Bypass: Vascular Anatomy and 5 Bypass Techniques.用于颅内-颅内搭桥的人胎盘模拟器:血管解剖与5种搭桥技术
World Neurosurg. 2018 Nov;119:e694-e702. doi: 10.1016/j.wneu.2018.07.246. Epub 2018 Aug 9.
3
Learning brain aneurysm microsurgical skills in a human placenta model: predictive validity.
一种用于通过血流和荧光成像训练和教授微血管吻合技术的动态4D显微外科旁路模拟器的验证
World Neurosurg X. 2024 Sep 21;24:100396. doi: 10.1016/j.wnsx.2024.100396. eCollection 2024 Oct.
J Neurosurg. 2018 Mar;128(3):846-852. doi: 10.3171/2016.10.JNS162083. Epub 2017 Mar 24.
4
Some anatomical observations on the human placenta as applied to microvascular surgical practice.关于人体胎盘应用于微血管外科手术实践的一些解剖学观察。
Br J Plast Surg. 1983 Jul;36(3):387-91. doi: 10.1016/s0007-1226(83)90066-8.
5
The development of a dynamic model for microvascular research and practice using human placenta: a preliminary report.利用人胎盘建立用于微血管研究与实践的动态模型:初步报告
Br J Plast Surg. 1985 Jul;38(3):389-93. doi: 10.1016/0007-1226(85)90248-6.