Madej A, Kindahl H, Larsson K, Edqvist L E
Acta Vet Scand. 1986;27(2):280-95. doi: 10.1186/BF03548172.
Peripheral plasma levels of 15-keto-13,14-dihydro-PGF, progesterone, Cortisol, LH and prolactin were studied in 6 primiparous postpartum dairy cows. The cows were followed by hormone measurements and clinical examinations from parturition until pregnancy was established. Blood was collected 3 times per day. The cervix, uterus and ovaries were examined by rectal palpation at 6–10 days intervals. The cows were observed for signs of oestrus twice daily and were additionally teased with a bull to provoke standing heat. Four cows had a normal parturition and dropped their fetal membranes shortly afterwards. (NR group). The remaining 2 retained their fetal membranes for more than 24 h following parturition (RFM group). One out of 6 cows showed standing oestrus at the first ovulation, 4 animals were in oestrus at the second ovulation and all cows showed signs of oestrus at the third ovulation. Although the length of the first luteal phase varied from 9 to 22 days a corpus luteum was in all cases palpated. The secretion of progesterone during the first luteal phase was terminated by a PGF release. A significant difference in 15-keto-13,14-dihydro-PGF levels between the 2 groups was found on days 0–4 (2.39 vs 6.87 nmol/1 at Ρ < 0.06). Postpartum prostaglandin F release as reflected by the level of 15-keto-13,14-dihydro-PGF lasted shorter in the NR group than in the RFM group (15–17 vs 21 days). Significant positive correlations between 15-keto-13,14-dihydro-PGF and Cortisol as well as between prolactin and Cortisol during the first 24 days postpartum were noted only in cows having normal parturition. The most pronounced daily prolactin variations occurred during the second luteal phase (NR group), when a significant difference between the times 8.00, 12.00 and 15.00 was recorded (14.7, 31.5 and 19.7 μg/l, respectively). Moreover, a partial negative correlation between log value of prolactin and arithmetical value of LH was found in these cows only during the first luteal phase after parturition.
对6头初产产后奶牛的外周血中15-酮-13,14-二氢-PGF、孕酮、皮质醇、促黄体生成素（LH）和催乳素水平进行了研究。从分娩到确定怀孕，对这些奶牛进行激素测量和临床检查。每天采集3次血液。每隔6 - 10天通过直肠触诊检查子宫颈、子宫和卵巢。每天观察奶牛两次发情迹象，并额外用公牛逗引以诱发站立发情。4头奶牛正常分娩，随后不久胎膜脱落（NR组）。其余2头奶牛在分娩后胎膜保留超过24小时（RFM组）。6头奶牛中有1头在首次排卵时出现站立发情，4头在第二次排卵时发情，所有奶牛在第三次排卵时均表现出发情迹象。尽管第一个黄体期的长度从9天到22天不等，但在所有情况下都能触诊到黄体。第一个黄体期孕酮的分泌因PGF释放而终止。在第0 - 4天，两组之间15-酮-13,14-二氢-PGF水平存在显著差异（分别为2.39和6.87 nmol/1，P < 0.06）。NR组中由15-酮-13,14-二氢-PGF水平反映的产后前列腺素F释放持续时间比RFM组短（15 - 17天对21天）。仅在正常分娩的奶牛中，产后前24天内观察到15-酮-13,14-二氢-PGF与皮质醇之间以及催乳素与皮质醇之间存在显著正相关。最明显的每日催乳素变化发生在第二个黄体期（NR组），此时记录到8.00、12.00和15.00时存在显著差异（分别为14.7、31.5和19.
