Shoily Sabrina Samad, Fatema Kaniz, Dina Rasheda Begum, Biswas Anik, Haque Papia, Rahman Mohammed Mizanur, Uddin Md Zulhash, Sajib Abu Ashfaqur
Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh.
Department of Wet Process Engineering, Bangladesh University of Textiles, Dhaka, Bangladesh.
J Genet Eng Biotechnol. 2023 Nov 23;21(1):136. doi: 10.1186/s43141-023-00596-5.
Enzymatic catalysis in different industrial applications is often preferred over chemical methods due to various advantages, such as higher specificity, greater efficiency, and less environmental footprint. Pectinases are a group of enzymes that catalyze the degradation of pectic compounds, the key components of plant middle lamella and the primary cell wall. Pectinases have found applications in multiple industrial processes, including cotton bioscouring, fruit juice extraction and its clarification, plant fiber degumming, paper making, plant biomass liquefaction, and saccharification, among others. The purpose of this study was to taxonomically characterize a bacterial species exhibiting pectinolytic activities and assess its pectinolytic activity qualitatively and quantitatively, as well as test its bioscouring potential.
Here, we report that Burkholderia cepacia, a previously unknown species with pectinolytic activity, exerts such activity comparable to commercially used pectinase enzymes in the textile industry, but requires less temperature for activity.
Quantitative evaluation of enzyme activity indicates the potential of the bacterial species for use in the bioscouring of cotton knit fabric.
在不同的工业应用中，酶催化由于具有多种优势，如更高的特异性、更高的效率和更小的环境影响，往往比化学方法更受青睐。果胶酶是一类催化果胶化合物降解的酶，果胶化合物是植物中层和初生细胞壁的关键成分。果胶酶已在多种工业过程中得到应用，包括棉织物生物精练、果汁提取与澄清、植物纤维脱胶、造纸、植物生物质液化和糖化等。本研究的目的是对一种具有果胶分解活性的细菌进行分类学表征，定性和定量评估其果胶分解活性，并测试其生物精练潜力。
在此，我们报告洋葱伯克霍尔德菌，一种先前未知的具有果胶分解活性的物种，其发挥的这种活性与纺织工业中商业使用的果胶酶相当，但活性所需温度更低。
酶活性的定量评估表明该细菌物种在棉针织物生物精练中的应用潜力。
