肺石棉纤维负荷分析：法医学评估计数规则的影响。
Lung asbestos fiber burden analysis: effects of the counting rules for legal medicine evaluations.
机构信息
Regional Electron Microscopy Centre-Environmental Protection Agency of Lombardy Region, Milan, Italy.
Formerly Occupational Health Unit, Local Health Authority and Mesothelioma Registry of Brescia, Brescia, Italy.
出版信息
Inhal Toxicol. 2023 Oct-Nov;35(11-12):300-307. doi: 10.1080/08958378.2023.2285789. Epub 2023 Dec 7.
OBJECTIVES
The work shows the effect of counting rules, such as analysis magnification and asbestos fiber dimension to be count (with length ≥5 µm or also asbestos fibers with length <5 µm) in the lung asbestos fiber burden analysis for legal medicine evaluations.
METHODS
On the same lung tissue samples, two different analyses were carried out to count any asbestos fibers with length ≥1 µm and with length ≥5 µm. Results of the amphibole burden of the two analyses were compared by linear regression analysis on log10-transformed values.
RESULTS
The analysis should be carried out at an appropriate magnification and on samples prepared in such a way as they allow the counting of very fine fibers. If the analysis is limited to the asbestos fibers with length ≥5 µm, there is a high risk of not detecting possible residual chrysotile fiber burden and thinner crocidolite asbestos fibers.
CONCLUSIONS
On average we estimated that 1 amphibole fiber with length ≥5 µm corresponds to ∼8 amphibole fibers with length ≥1 µm in the lung. The values of the Helsinki criteria should be updated taking this into account.
目的
本工作展示了计数规则的效果，例如分析放大倍数和石棉纤维尺寸（长度≥5μm 或长度<5μm 的石棉纤维）在法医评估肺内石棉纤维负荷分析中的作用。
方法
对同一肺组织样本进行了两种不同的分析，以计数长度≥1μm 和长度≥5μm 的任何石棉纤维。通过对对数转换值进行线性回归分析，比较了两种分析的角闪石负荷结果。
结果
分析应在适当的放大倍数下进行，并在制备样本时应允许计数非常细的纤维。如果分析仅限于长度≥5μm 的石棉纤维，则存在无法检测可能残留的温石棉纤维负荷和更细的青石棉纤维的高风险。
结论
平均而言，我们估计肺内长度≥5μm 的 1 根角闪石纤维相当于长度≥1μm 的约 8 根角闪石纤维。应考虑到这一点，更新赫尔辛基标准的值。
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