Cellular and Molecular Research Center, Research Institute for Prevention of Non-Commuicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran.
Department of Chemistry, Imam Khomeini International University, Qazvin, Iran.
Biochem Biophys Res Commun. 2024 Jan 1;690:149219. doi: 10.1016/j.bbrc.2023.149219. Epub 2023 Nov 19.
There has been a growth in the use of plant compounds as biological products for the prevention and treatment of various diseases, including cancer. As a phenolic compound, p-Coumaric acid (p-CA) demonstrates preferrable biological effects such as anti-cancer activities. A nano-liposomal carrier containing p-CA was designed to increase the anticancer effectiveness of this compound on melanoma cells (A375). To determine the characteristics of synthesized liposomes, encapsulation efficiency was measured. In addition, the particle size was measured utilizing DLS, FTIR, and morphology examination using SEM. In vitro release was also studied through the dialysis method, while toxicity was evaluated using the MTT assay. To determine apoptotic characteristics, biotechnology tools like flow cytometry, real time PCR, and atomic force microscopy (AFM) were employed. The findings indicated that in the cells treated with the liposomal form of p-CA, the amount of elastic modulus was higher compared to its free form. Kinetic modeling indicated that the best fitting model was zero-order.
植物化合物作为预防和治疗各种疾病（包括癌症）的生物制品的使用有所增加。对香豆酸（p-CA）作为一种酚类化合物，具有较好的生物效应，如抗癌活性。设计了一种包含 p-CA 的纳米脂质体载体，以提高该化合物对黑色素瘤细胞（A375）的抗癌效果。为了确定合成脂质体的特性，测量了包封效率。此外，还利用 DLS、FTIR 和 SEM 形貌检查测量了粒径。还通过透析法研究了体外释放，并用 MTT 测定法评估了毒性。为了确定凋亡特征，使用了生物技术工具，如流式细胞术、实时 PCR 和原子力显微镜（AFM）。结果表明，在用 p-CA 的脂质体形式处理的细胞中，弹性模量的量高于其游离形式。动力学模型表明，最佳拟合模型是零级。
