• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

维生素D与黑色素生成抑制：脂质体维生素D纳米载体对B16 F10细胞系抗黑色素生成活性的研究

Vitamin D and Inhibition of Melanogenesis: Examination of Liposomal Vitamin D Nanocarrier Anti-Melanogenesis Activity on B16 F10 Cell Line.

作者信息

Bahrami Azita, Farasat Alireza, Zolghadr Leila, Sabaghi Yalda, Gheibi Nematollah

机构信息

Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Diseases Qazvin University of Medical Sciences Qazvin Iran.

Monoclonal Antibody Research Center Avicenna Research Institute, ACECR Tehran Iran.

出版信息

Food Sci Nutr. 2025 May 19;13(5):e70302. doi: 10.1002/fsn3.70302. eCollection 2025 May.

DOI:10.1002/fsn3.70302
PMID:40390845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12086370/
Abstract

Despite various treatment strategies employed thus far, malignant melanoma usually resists most of the interventions. The present work is an attempt to synthesize liposomes encapsulating vitamin D, evaluate their physicochemical properties, evaluate the release kinetics of vitamin D, and examine the cytotoxicity of both liposomal and free vitamin D on melanoma cells in vitro. The analysis revealed that the zero-order model exhibited the best correlation (0.998) at acidic pH (5.5), indicating the most efficient vitamin D release from the nanoparticles. The results indicated that the IC50 values for cancer cells exposed to vitamin D-containing liposomes were 35.08 and 28.96 μg/mL after 24-48 h. Compared to those treated with free vitamin D, flow cytometry results further demonstrated a higher apoptosis in B16 F10 cells exposed to vitamin D-containing liposomes. Moreover, vitamin D-containing liposomes induced the most significant nanomechanical alterations, with the most pronounced decrease in the expression of the PI3K/AKT1 gene observed in the liposomal vitamin D treatment group.

摘要

尽管迄今为止采用了各种治疗策略，但恶性黑色素瘤通常对大多数干预措施具有抗性。本研究旨在合成包裹维生素D的脂质体，评估其物理化学性质，评估维生素D的释放动力学，并检测脂质体维生素D和游离维生素D对黑色素瘤细胞的体外细胞毒性。分析表明，零级模型在酸性pH值（5.5）下具有最佳相关性（0.998），表明纳米颗粒中维生素D的释放效率最高。结果表明，暴露于含维生素D脂质体的癌细胞在24 - 48小时后的IC50值分别为35.08和28.96μg/mL。与游离维生素D处理的细胞相比，流式细胞术结果进一步表明，暴露于含维生素D脂质体的B16 F10细胞凋亡率更高。此外，含维生素D脂质体引起了最显著的纳米力学改变，在脂质体维生素D治疗组中观察到PI3K/AKT1基因表达下降最为明显。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/a6f2e4f1c091/FSN3-13-e70302-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/27347ed017eb/FSN3-13-e70302-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/3d495844c86f/FSN3-13-e70302-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/95dc9b485c4d/FSN3-13-e70302-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/c2630a99fcbc/FSN3-13-e70302-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/0dd19c3fc22b/FSN3-13-e70302-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/7dd7aeea3a76/FSN3-13-e70302-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/e40cc6b95de2/FSN3-13-e70302-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/5190d69dbbe5/FSN3-13-e70302-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/a6f2e4f1c091/FSN3-13-e70302-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/27347ed017eb/FSN3-13-e70302-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/3d495844c86f/FSN3-13-e70302-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/95dc9b485c4d/FSN3-13-e70302-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/c2630a99fcbc/FSN3-13-e70302-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/0dd19c3fc22b/FSN3-13-e70302-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/7dd7aeea3a76/FSN3-13-e70302-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/e40cc6b95de2/FSN3-13-e70302-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/5190d69dbbe5/FSN3-13-e70302-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a446/12086370/a6f2e4f1c091/FSN3-13-e70302-g004.jpg

相似文献

1
Vitamin D and Inhibition of Melanogenesis: Examination of Liposomal Vitamin D Nanocarrier Anti-Melanogenesis Activity on B16 F10 Cell Line.维生素D与黑色素生成抑制：脂质体维生素D纳米载体对B16 F10细胞系抗黑色素生成活性的研究
Food Sci Nutr. 2025 May 19;13(5):e70302. doi: 10.1002/fsn3.70302. eCollection 2025 May.
2
The anticancer impacts of free and liposomal caffeic acid phenethyl ester (CAPE) on melanoma cell line (A375).游离型和脂质体型绿原酸咖啡酸酯（CAPE）对黑素瘤细胞系（A375）的抗癌作用。
Cell Biochem Funct. 2024 Jan;42(1):e3900. doi: 10.1002/cbf.3900. Epub 2023 Dec 18.
3
Antitumor effect of iRGD-modified liposomes containing conjugated linoleic acid-paclitaxel (CLA-PTX) on B16-F10 melanoma.载共轭亚油酸-紫杉醇（CLA-PTX）的 iRGD 修饰脂质体对 B16-F10 黑色素瘤的抗肿瘤作用。
Int J Nanomedicine. 2014 Jun 24;9:3091-105. doi: 10.2147/IJN.S65664. eCollection 2014.
4
A nano-liposomal carrier containing p-coumaric acid for induction of targeted apoptosis on melanoma cells and kinetic modeling.一种载有对香豆酸的纳米脂质体载体，用于诱导黑素瘤细胞的靶向细胞凋亡及动力学建模。
Biochem Biophys Res Commun. 2024 Jan 1;690:149219. doi: 10.1016/j.bbrc.2023.149219. Epub 2023 Nov 19.
5
A Newly Authenticated Compound from Traditional Chinese Medicine Decoction Induces Melanogenesis in B16-F10 Cells by Increasing Tyrosinase Activity.一种新鉴定出的来自中药汤剂的化合物通过提高酪氨酸酶活性诱导B16-F10细胞黑色素生成。
Evid Based Complement Alternat Med. 2018 Nov 18;2018:8485670. doi: 10.1155/2018/8485670. eCollection 2018.
6
In vitro effects of l-kynurenine and quinolinic acid on adhesion, migration and apoptosis in B16 F10 melanoma cells.L-犬尿氨酸和喹啉酸对 B16F10 黑素瘤细胞黏附、迁移和凋亡的体外影响。
Biochem Biophys Res Commun. 2024 Dec 3;736:150851. doi: 10.1016/j.bbrc.2024.150851. Epub 2024 Oct 18.
7
Celastrol inhibits mouse B16-F10 melanoma cell survival by regulating the PI3K/AKT/mTOR signaling pathway and repressing HIF-1α expression.雷公藤红素通过调节PI3K/AKT/mTOR信号通路并抑制HIF-1α表达来抑制小鼠B16-F10黑色素瘤细胞的存活。
Discov Oncol. 2024 May 21;15(1):178. doi: 10.1007/s12672-024-01045-6.
8
The antitumor activity of a doxorubicin loaded, iRGD-modified sterically-stabilized liposome on B16-F10 melanoma cells: in vitro and in vivo evaluation.载多柔比星、iRGD 修饰的立体稳定脂质体对 B16-F10 黑素瘤细胞的抗肿瘤活性：体外和体内评价。
Int J Nanomedicine. 2013;8:2473-85. doi: 10.2147/IJN.S46962. Epub 2013 Jul 15.
9
Anti-Melanogenic Effect of Ethanolic Extract of on IBMX-Induced Melanogenesis in B16/F10 Melanoma Cells.对 IBMX 诱导的 B16/F10 黑素瘤细胞黑素生成的醇提物的抗黑素生成作用。
Nutrients. 2020 Mar 20;12(3):832. doi: 10.3390/nu12030832.
10
Antitumor Assessment of Liposomal Beta-Carotene with Tamoxifen Against Breast Carcinoma Cell Line: An In Vitro Study.脂质体β-胡萝卜素联合他莫昔芬对乳腺癌细胞系的抗肿瘤评估：一项体外研究。
Biomolecules. 2025 Mar 26;15(4):486. doi: 10.3390/biom15040486.

本文引用的文献

1
A nano-liposomal carrier containing p-coumaric acid for induction of targeted apoptosis on melanoma cells and kinetic modeling.一种载有对香豆酸的纳米脂质体载体，用于诱导黑素瘤细胞的靶向细胞凋亡及动力学建模。
Biochem Biophys Res Commun. 2024 Jan 1;690:149219. doi: 10.1016/j.bbrc.2023.149219. Epub 2023 Nov 19.
2
Activation of apoptosis and G0/G1 cell cycle arrest along with inhibition of melanogenesis by humic acid and fulvic acid: -2 and genes expression and evaluation of nanomechanical properties in A375 human melanoma cell line.腐殖酸和富里酸诱导细胞凋亡、使细胞周期停滞于G0/G1期并抑制黑色素生成：A375人黑色素瘤细胞系中-2和基因表达及纳米力学性能评估
Iran J Basic Med Sci. 2022 Apr;25(4):489-496. doi: 10.22038/IJBMS.2022.60651.13444.
3
PTEN: A novel target for vitamin D in melanoma.PTEN：维生素 D 在黑色素瘤中的新靶点。
J Steroid Biochem Mol Biol. 2022 Apr;218:106059. doi: 10.1016/j.jsbmb.2022.106059. Epub 2022 Jan 13.
4
Metastatic uveal melanoma: The final frontier.转移性葡萄膜黑素瘤：最后的前沿。
Prog Retin Eye Res. 2022 Sep;90:101041. doi: 10.1016/j.preteyeres.2022.101041. Epub 2022 Jan 6.
5
A review on liposome-based therapeutic approaches against malignant melanoma.脂质体治疗恶性黑色素瘤的研究进展。
Int J Pharm. 2021 Apr 15;599:120413. doi: 10.1016/j.ijpharm.2021.120413. Epub 2021 Mar 2.
6
Overcoming barriers by local drug delivery with liposomes.通过脂质体实现局部药物递送以克服障碍。
Adv Drug Deliv Rev. 2021 Jul;174:53-86. doi: 10.1016/j.addr.2021.01.019. Epub 2021 Feb 2.
7
Vitamin D and its low calcemic analogs modulate the anticancer properties of cisplatin and dacarbazine in the human melanoma A375 cell line.维生素 D 及其低钙类似物可调节顺铂和达卡巴嗪在人黑色素瘤 A375 细胞系中的抗癌特性。
Int J Oncol. 2019 Apr;54(4):1481-1495. doi: 10.3892/ijo.2019.4725. Epub 2019 Feb 25.
8
Lipids and polymers in pharmaceutical technology: Lifelong companions.药物制剂技术中的脂质和聚合物：相伴终生。
Int J Pharm. 2019 Mar 10;558:128-142. doi: 10.1016/j.ijpharm.2018.12.080. Epub 2019 Jan 9.
9
Role of Vitamin D Beyond the Skeletal Function: A Review of the Molecular and Clinical Studies.维生素 D 作用的超越：骨骼功能以外的分子和临床研究综述。
Int J Mol Sci. 2018 May 30;19(6):1618. doi: 10.3390/ijms19061618.
10
Inhalable particulate drug delivery systems for lung cancer therapy: Nanoparticles, microparticles, nanocomposites and nanoaggregates.可吸入颗粒药物传递系统治疗肺癌：纳米粒子、微粒子、纳米复合材料和纳米聚集体。
J Control Release. 2018 Jan 10;269:374-392. doi: 10.1016/j.jconrel.2017.11.036. Epub 2017 Nov 24.